Cdc13 exhibits dynamic DNA strand exchange in the presence of telomeric DNA

• Published in: bioRxiv
• Main Authors: Nickens, David G, Feng, Zhitong, Shen, Jiangchuan, Gray, Spencer J, Simmons, Robert H, Niu, Hengyao, Bochman, Matthew L
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 28.02.2024
• Subjects: Affinity; Binding sites; DNA helicase; Genomes; Nucleotide sequence; Single-stranded DNA; Telomerase; Telomeres; Yeast
• DOI: 10.1101/2023.12.04.569902

Telomerase is the enzyme that lengthens telomeres and is tightly regulated by a variety of means to maintain genome integrity. Several DNA helicases function at telomeres, and we previously found that the Saccharomyces cerevisiae helicases Hrq1 and Pif1 directly regulate telomerase. To extend these findings, we are investigating the interplay between helicases, single-stranded DNA (ssDNA) binding proteins (ssBPs), and telomerase. The yeast ssBPs Cdc13 and RPA differentially affect Hrq1 and Pif1 helicase activity, and experiments to measure helicase disruption of Cdc13/ssDNA complexes instead revealed that Cdc13 can exchange between substrates. Although other ssBPs display dynamic binding, this was unexpected with Cdc13 due to the reported in vitro stability of the Cdc13/telomeric ssDNA complex. We found that the DNA exchange by Cdc13 occurs rapidly at physiological temperatures, requires telomeric repeat sequence DNA, and is affected by ssDNA length. Cdc13 truncations revealed that the low-affinity binding site (OB1), which is distal from the high-affinity binding site (OB3), is required for this intermolecular dynamic DNA exchange (DDE). We hypothesize that DDE by Cdc13 is the basis for how Cdc13 'moves' at telomeres to alternate between modes where it regulates telomerase activity and assists in telomere replication.Competing Interest StatementThe authors have declared no competing interest.Footnotes* Main text, supplemental materials, and figures