IL-10 promotes ocular neovascularization by regulating macrophages

• Published in: Shanghai jiao tong da xue xue bao. Yi xue ban Vol. 37; no. 3
• Main Authors: Sui, Ai-ling, Su, Ting, Gao, Yu-shuo, Zhu, Yan-ji, Xie, Bing
• Format: Journal Article
• Language: Chinese
• Published: 01.03.2017
• ISSN: 1674-8115
• DOI: 10.3969/j.issn.1674-8115.2017.03.002

Objective [middot] To investigate the role of interleukin-10(IL-10) in regulating ocular neovascularization(NV). Methods [middot] Expression of IL-10 was investigated in mice with oxygen-induced retinopathy(OIR) and transgenic mice with VEGF expression in photoreceptors by immunofluorescence, RT-PCR, and Western blotting. Mice deficient in IL-10 were used to test the effect of IL-10 in retinal, sub-retinal, and choroidal NV. Results [middot] In OIR mice and transgenic mice with VEGF expression in photoreceptors, the staining intensity and m RNA expression of IL-10 were increased. Mice deficient in IL-10 showed a significant reduction in ischemia-induced retinal NV, and choroidal NV at rupture sites in Bruch's membrane. Mice lacking IL-10 showed reduced levels of hypoxia-inducible factor-1[alpha](HIF-1[alpha]) and suppression of ischemia-induced expression of VEGF and VEGF receptor 1. Macrophage was regulated and reduced in ischemic retina of mice with IL-10 deficiency. Conclusion [middot] IL-10 stimulates ocu