Analysis of biochemical reactions of Porphyromonas gingivalis clinical isolates
Objective To detect the biochemical reactions and analyze the carbohydrate metabolism of Porphyromonas gingivalis (P. gingivalis) clinical isolates, with type strains as controls. Methods API 20A and ATB Rapid ID 32A biochemical reaction testing systems were used to determine the biochemical metabol...
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Published in | Shanghai jiao tong da xue xue bao. Yi xue ban Vol. 33; no. 2; pp. 168 - 173 |
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Main Authors | , , , |
Format | Journal Article |
Language | Chinese |
Published |
01.02.2013
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Subjects | |
Online Access | Get full text |
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Summary: | Objective To detect the biochemical reactions and analyze the carbohydrate metabolism of Porphyromonas gingivalis (P. gingivalis) clinical isolates, with type strains as controls. Methods API 20A and ATB Rapid ID 32A biochemical reaction testing systems were used to determine the biochemical metabolic properties of P. gingivalis clinical isolates (SJD2, SJD4, SJD5, SJD11 and SJD12), with type strains (W83 and ATCC 33277) as controls. Results All tested P. gingivalis strains possessed enzymatic activity of beta -galactosidase and beta -N-Acetyl-glucosaminidase. These strains had capacities to hydrolyse several amino acid residues and free amino acids such as tryptophan and arginine. In addition, there existed heterogeneity in the biochemical reactions among P. gingivalis strains. Furthermore, different bacterial identification results were found between API 20A biochemical reaction testing system and ATB Rapid ID 32A biochemical reaction testing system. Conclusion P. gingivalis may utilize peptides and amino a |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 1674-8115 |
DOI: | 10.3969/j.issn.1674-8115.2013.02.008 |