Effects of long non-coding RNA URHC on proliferation, apoptosis and invasion of colorectal cancer cells
OBJECTIVETo investigate the effect of long non-coding RNA URHC on the proliferation, apoptosis and invasion of colorectal cancer cells. PATIENTS AND METHODSThe expression of lncRNA-URHC in tissues and cells was tested by Real-time quantitative PCR. The expression of lncRNA-URHC was down-regulated by...
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Published in | European review for medical and pharmacological sciences Vol. 22; no. 6; pp. 1658 - 1664 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
2021
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Online Access | Get more information |
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Summary: | OBJECTIVETo investigate the effect of long non-coding RNA URHC on the proliferation, apoptosis and invasion of colorectal cancer cells. PATIENTS AND METHODSThe expression of lncRNA-URHC in tissues and cells was tested by Real-time quantitative PCR. The expression of lncRNA-URHC was down-regulated by RNA interference (siRNA). The Real-time quantitative polymerase chain reaction (PCR) method was used to detect the interference efficiency. Cell counting kit-8 (CCK-8), flow cytometry, and transwell were used to detect the effect of lncRNA-URHC on the proliferation, apoptosis and invasion of colorectal cancer cells. The effect of lncRNA-URHC on epithelial-mesenchymal transition (EMT)-related markers was detected by Western blot. RESULTSLncRNA-URHC expression was significantly increased in colorectal cancer cells compared with normal cells, and the expression of lncRNA-URHC in colorectal cancer cells was higher than that in the normal cell. After down-regulated the expression of lncRNA-URHC, the proliferation and invasion of colorectal cancer cells were decreased, while cells apoptosis was promoted. Down-regulation of lncRNA-URHC could enhance the expression of E-cadherin and reduce the expression of N-cadherin, vimentin and snail. CONCLUSIONSDown-regulation of lncRNA-URHC can inhibit the progression of colorectal cancer. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Correction/Retraction-1 ObjectType-Feature-3 content type line 23 |
ISSN: | 2284-0729 |
DOI: | 10.26355/eurrev_201803_14577 |