Preliminary study on lung cancer rat model produced by Chlamydia pneumoniae (Cpn)

• Published in: Fu dan xue bao. Fudan University journal of medical science. Yi xue ban Vol. 39; no. 5; pp. 520 - 524
• Main Authors: Chu, D-J, Guo, S-G, Pan, C-F, Wang, J, Du, Y, Yu, Z-Y
• Format: Journal Article
• Language: Chinese
• Published: 01.09.2012
• Subjects: Animal models; benzopyrene; Chlamydophila pneumoniae; Enzyme-linked immunosorbent assay; Infection; Lung cancer; Lung carcinoma; Polymerase chain reaction; Trachea
• ISSN: 1672-8467
• DOI: 10.3969/j.issn.1672-8467.2012.05.016

Objective Preliminary study on rat model of lung cancer produced by Chlamydia pneumoniae (Cpn). Methods The lung cancer animal model was developed by intratracheal repeated injecting Cpn (TW-183) into lung of rat. The Cpn-antibodies (Cpn-IgA, -IgG and -IgM) of rat serum were measured by microimmunofluorescence (MIF) method. The Cpn-DNA or Cpn-Ag of rat lung cancer were detected by PCR method or ELISA. Results The prevalence of Cpn infection was 72.9% (35/48) in Cpn group, and 76.7% (33/43) in Cpn plus benzopyrene (Bp) group. The incidences of lung carcinomas of the two groups were 25.0% (12/48) and 44.2% (19/43). Compared with normal controls, P value were 0.001 and 0.000, respectively. Coodnsions A rat model of lung carcinoma produced by Cpn was successfully prepared for further study in experiment on treatment, prevention and mechanism.