Characters of VP1 gene sequence of a Coxsackievirus B2 isolate

• Published in: Zhongguo sheng wu zhi pin xue za zhi Vol. 28; no. 1; pp. 49 - 53
• Main Authors: SU, Ting, ZHU, Yan-ju, YAN, Shan-shan, CHEN, Jun-ying, YANG, Shun-qiao, PAN, Yue, CHEN, Wei, SHAO, Cong-wen, MA, Shao-hui
• Format: Journal Article
• Language: Chinese
• Published: 20.01.2015
• Subjects: Coxsackievirus
• ISSN: 1004-5503

Objective To analyze the characters of complete VP1 gene of Coxsackievirus B2 (CVB2) isolate. Methods Virus was isolated from 10 fecal samples of suspected children with hand-foot-mouth disease (HFMD) with RD cells, from which VPl gene was amplified by RT-PCR and sequenced. The sequencing results were analyzed by using NCBI BLAST, Mega 6.1 and Geneious software, based on which a phylogenetic tree was constructed. Results A CVB2 strain was isolated from 10 fecal samples of suspected children with HFMD and named as 509/YN/CHN/2010, of which the full-length of VP1 nucleotide, 846 bp, was consistent with those of other CVB2 strains. The homologies of nucleotide and amino acid sequences of the isolate were 81.0% ~ 95.3% and 97.9% ~ 98.9% to those of other strains isolated in China, and 81.1% - 87.8% and 97.9% - 98.6% to those isolated abroad, respectively. Phylogenetic analysis showed that the isolate belonged to two distinct clusters with other isolates from China. The evolutional relationship of Coxsackievirus B