Screening, Identification and Security Test of Camellia oleifera Rhizosphere Phosphate-Solubilizing Bacteria

• Published in: Forest research (Beijing) Vol. 28; no. 2
• Main Authors: Wang, Shu, Zhang, Lin-ping, Hao, Fei-fei, Zhang, Yang, Hu, Dong-nan
• Format: Journal Article
• Language: Chinese
• Published: 01.04.2015
• Subjects: Allium cepa; Bacillus; Camellia; Medicago sativa
• ISSN: 1001-1498

Twenty strains of inorganic phosphate-solubilizing bacteria were isolated from Camellia oleifera rhizosphere using phosphate-solubilizing circle and the molybdenum--anti-spectrophotometric method. The phosphate-solubilizing capacity was investigated from 20 strains based on qualitative and quantitative test. The results showed that there was a significant negative correlation between the phosphate solubilizing capacity and the p H medium (P < 0.01), whereas there was no such phosphate solubilizing capacity on D/d. The strain NC285 was identified by its morphological, physiological and biochemical characteristics, Biolog system and 16 Sr DNA gene sequence analysis. The high effective phosphate-solubilizing bacterium of NC285 was identified as Bacillus aryabhattai. The virulence of NC285 was tested on Allium cepa and Medicago sativa seedlings, and the cbl A virulence genes. No cbl A gene was detected on NC285 in the test, so this strain is safe for A. cepa and M. sativa.