Expression of HIV-1 epitopes included in particles formed by human hepatitis B virus nucleocapsid protein
Hybrids of the core protein of hepatitis B virus (HBcAg) have been designed which carry N-terminal insertions of B- and T-cell epitopes of HIV-1 an immunodominant B-epitope from gp41, a T-cell epitope from p34 pol, and a cluster of B- and T-cell epitopes from p17 gag. The hybrids have been synthesiz...
Saved in:
Published in | Biokhimiia (Moscow, Russia) Vol. 61; no. 3; p. 532 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | Russian |
Published |
Russia (Federation)
01.03.1996
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Summary: | Hybrids of the core protein of hepatitis B virus (HBcAg) have been designed which carry N-terminal insertions of B- and T-cell epitopes of HIV-1 an immunodominant B-epitope from gp41, a T-cell epitope from p34 pol, and a cluster of B- and T-cell epitopes from p17 gag. The hybrids have been synthesized using two expression systems-one based on the thermoinducible PR promoter of bacteriophage lambda and the other one based on phi 10 promoter of bacteriophage T7 with 3-5% and 7-14% yields, respectively. The hybrids have dual HBV and HIV-1 immunospecificity and are assembled into particles similar to those formed by the protein carrier HBcAg. Sandwich ELISA and immune electron microscopy revealed that HIV-1 epitopes are exposed on the surface of the particles. |
---|---|
ISSN: | 0320-9725 |