Expression of HIV-1 epitopes included in particles formed by human hepatitis B virus nucleocapsid protein

Hybrids of the core protein of hepatitis B virus (HBcAg) have been designed which carry N-terminal insertions of B- and T-cell epitopes of HIV-1 an immunodominant B-epitope from gp41, a T-cell epitope from p34 pol, and a cluster of B- and T-cell epitopes from p17 gag. The hybrids have been synthesiz...

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Bibliographic Details
Published inBiokhimiia (Moscow, Russia) Vol. 61; no. 3; p. 532
Main Authors Isaguliants, M G, Kadoshnikov, Iu P, Kalinina, T I, Khuliakov, Iu E, Semiletov, Iu A, Smirnov, V D, Wahren, B
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.03.1996
Subjects
Amino Acid Sequence
Bacteriophage lambda - genetics
Base Sequence
Cloning, Molecular
DNA, Recombinant
Epitopes - genetics
Hepatitis B Core Antigens - genetics
HIV-1 - immunology
Humans
Microscopy, Electron
Molecular Sequence Data
Plasmids
Promoter Regions, Genetic
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - ultrastructure
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Summary:Hybrids of the core protein of hepatitis B virus (HBcAg) have been designed which carry N-terminal insertions of B- and T-cell epitopes of HIV-1 an immunodominant B-epitope from gp41, a T-cell epitope from p34 pol, and a cluster of B- and T-cell epitopes from p17 gag. The hybrids have been synthesized using two expression systems-one based on the thermoinducible PR promoter of bacteriophage lambda and the other one based on phi 10 promoter of bacteriophage T7 with 3-5% and 7-14% yields, respectively. The hybrids have dual HBV and HIV-1 immunospecificity and are assembled into particles similar to those formed by the protein carrier HBcAg. Sandwich ELISA and immune electron microscopy revealed that HIV-1 epitopes are exposed on the surface of the particles.
ISSN:0320-9725