Development of a multireactor microfluidic system for the determination of DNA using real-time polymerase chain reaction

• Published in: Journal of analytical chemistry (New York, N.Y.) Vol. 63; no. 2; pp. 192 - 198
• Main Authors: Slyadnev, M N, Lavrova, M V, Erkin, MA, Kazakov, V A, Ganeev, A A
• Format: Journal Article
• Language: English
• Published: 01.02.2008
• ISSN: 1061-9348; 1608-3199
• DOI: 10.1007/s10809-008-2015-2

A microfluidic system that allowed us to perform the real-time polymerase chain reaction (PCR) in a glass-silicon microchip containing nine 250-nL microreactors was developed and studied. The resulting high heating/cooling rates of a PCR mixture in a microreactor allowed us to optimize the amplification mode (1 min/cycle). The silicon surface of microreactors was successfully passivated. The resulting analytical system allowed us to measure the PCR kinetic curves in chip microreactors at a DNA concentration of similar to 5 x 10 super(4) copies per microreactor. It was found that, if the PCR is performed in a microchip with real-time detection using the optimized amplification mode, the result can be obtained 13-14 min after the onset of reaction.