Low-resolution H spin-spin relaxation of n-decane/water emulsions stabilized by beta -casein

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 48; no. 2; pp. 119 - 127
• Main Authors: Barros, Clarice N, Areas, Elizabeth PG, Figueiredo, Eliane N, Areas, Jose AG
• Format: Journal Article
• Language: English
• Published: 15.03.2006
• ISSN: 0927-7765
• DOI: 10.1016/j.colsurfb.2006.01.016

A low-resolution H NMR relaxometry study on the dynamics of an n- decane/water emulsion stabilized by beta -casein is presented. Spin-spin (transverse) relaxation time constants (T sub(2)) were used to assess relative mobilities of emulsion components, by a selective deuteration procedure. Data analysis allowed the emulsion investigated to be described by a heterogeneous collection of dynamically distinct populations. A major population of n- decane molecules presented an average mobility that very nearly approached that of pure solvent, which is compatible with its occurrence in the emulsion continuous microphase. beta -Casein molecules displayed a prevalent population with significantly decreased mobility as compared to the free protein in solution, which is in accordance with the protein location at the oil/water interface. Also, a major H sub(2)O population with significantly lower average T sub(2) as compared to the pure liquid was detected and has been assigned to interfacial water.