The early days of ex vivo 1H, 13C, and 31P nuclear magnetic resonance in the laboratory of Dr. Robert G. Shulman from 1975 to 1995

• Published in: NMR in biomedicine Vol. 36; no. 4
• Main Authors: Rothman, Douglas L., Behar, Kevin L., Petroff, Ognen A. C., Shulman, Robert G.
• Format: Journal Article
• Language: English
• Published: Oxford Wiley Subscription Services, Inc 01.04.2023
• Subjects: 13C MRS; 1H MRS; 31P MRS; Biological products; In vivo methods and tests; Laboratories; Magnetic resonance spectroscopy; metabolism; Microorganisms; NMR; Nuclear magnetic resonance; Quantitation; resonance assignment
• ISSN: 0952-3480; 1099-1492
• DOI: 10.1002/nbm.4879

This paper provides a brief description of the early use of ex vivo nuclear magnetic resonance (NMR) studies of tissue and tissue extracts performed in the laboratory of Dr. Robert G. Shulman from 1975 through 1995 at Bell Laboratories, then later at Yale University. During that period, ex vivo NMR provided critical information in support of resonance assignments and the quantitation of concentrations for magnetic resonance spectroscopy studies. The period covered saw rapid advances in magnet technology, starting with studies of microorganisms in vertical bore high‐resolution NMR studies, then by 1981 studies of small mammals in a horizontal bore magnet, and then studies of humans in 1984. Ex vivo NMR played a critical role in all these studies. A general strategy developed in the lab for using ex vivo NMR to support in vivo studies is presented, as well as illustrative examples. This paper provides a brief description of the early use of ex vivo NMR studies of tissue and tissue extracts performed in the laboratory of Dr. Robert G. Shulman from 1975 through 1995 at Bell Laboratories, then later at Yale University. During that period ex vivo NMR provided critical information in support of resonance assignments and the quantitation of concentrations for MRS studies. The period covered saw rapid advances in magnet technology, with the earliest studies in microorganisms in vertical bore high‐resolution NMR studies, with studies after 1984 in humans and small animals in large‐bore horizontal MRS/MRI scanners. A general strategy developed in the lab for using ex vivo NMR to support in vivo studies is presented, as well as illustrative examples. The figures shows in vivo (c), ex vivo (b), and extract (a) spectra from the first 1H MRS study of the brain in 1983.