Inactivation of the flagellin gene of Salmonella enterica serotype Enteritidis strongly reduces into differentiated Caco-2 cells

Abstract A nonflagellated mutant of Salmonella enterica serotype Enteritidis was constructed by disrupting the flagellin gene (fliC). Northern blot analysis indicated that the mutation did not affect expression of the downstream fliU gene. Infection experiments with differentiated Caco-2 cells revea...

Full description

Saved in:
Bibliographic Details
Published inFEMS microbiology letters Vol. 185; no. 2; pp. 175 - 179
Main Authors Van Asten, Fons J.A.M., Hendriks, Henno G.C.J.M., Koninkx, Jos F.J.G., van der Zeijst, Bernard A.M., Gaastra, Wim
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.04.2000
Oxford University Press
Subjects
Cell differentiation
Complementation
Deactivation
Disruption
Flagella
Flagellin
Gene expression
Inactivation
Microbiology
Mutation
Salmonella
Salmonella enterica
serotype Enteritidis
Flagella
Adhesion
Invasion
Caco-2 cell
Online AccessGet full text

Cover

More Information
Summary:Abstract A nonflagellated mutant of Salmonella enterica serotype Enteritidis was constructed by disrupting the flagellin gene (fliC). Northern blot analysis indicated that the mutation did not affect expression of the downstream fliU gene. Infection experiments with differentiated Caco-2 cells revealed that the mutant was about 50-fold less invasive than the wild-type strain, while bacterial adherence was unaffected. Complementation of the mutant with an intact fliC copy restored flagella formation and efficient bacterial. Our data demonstrate that the fliC gene of S. enterica serotype Enteritidis is essential for the of Caco-2 cells.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2000.tb09058.x