Trading water for carbon in the future: Effects of elevated CO2 and warming on leaf hydraulic traits in a semiarid grassland

• Published in: Global change biology Vol. 28; no. 20; pp. 5991 - 6001
• Main Authors: Mueller, Kevin E., Ocheltree, Troy W., Kray, Julie A., Bushey, Julie A., Blumenthal, Dana M., Williams, David G., Pendall, Elise
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell Publishing Ltd 01.10.2022; Wiley
• Subjects: Avoidance; Biodiversity & Conservation; Carbon dioxide; Climate change; Climate effects; Dominant species; Drought; Drought resistance; Environmental Sciences & Ecology; Forbs; Grasslands; Hydraulics; Interspecific; intraspecific; Leaf area; Leaves; Moisture effects; Phenotypes; Phenotypic plasticity; plant functional type; Plants; Plants (botany); Soil; Soil moisture; species; Stomata; Turgor; turgor loss point; warming; Water potential; Xylem
• ISSN: 1354-1013; 1365-2486
• DOI: 10.1111/gcb.16314

The effects of climate change on plants and ecosystems are mediated by plant hydraulic traits, including interspecific and intraspecific variability of trait phenotypes. Yet, integrative and realistic studies of hydraulic traits and climate change are rare. In a semiarid grassland, we assessed the response of several plant hydraulic traits to elevated CO2 (+200 ppm) and warming (+1.5 to 3°C; day to night). For leaves of five dominant species (three graminoids and two forbs), and in replicated plots exposed to 7 years of elevated CO2, warming, or ambient climate, we measured: stomatal density and size, xylem vessel size, turgor loss point, and water potential (pre‐dawn). Interspecific differences in hydraulic traits were larger than intraspecific shifts induced by elevated CO2 and/or warming. Effects of elevated CO2 were greater than effects of warming, and interactions between treatments were weak or not detected. The forbs showed little phenotypic plasticity. The graminoids had leaf water potentials and turgor loss points that were 10% to 50% less negative under elevated CO2; thus, climate change might cause these species to adjust their drought resistance strategy away from tolerance and toward avoidance. The C4 grass also reduced allocation of leaf area to stomata under elevated CO2, which helps explain observations of higher soil moisture. The shifts in hydraulic traits under elevated CO2 were not, however, simply due to higher soil moisture. Integration of our results with others' indicates that common species in this grassland are more likely to adjust stomatal aperture in response to near‐term climate change, rather than anatomical traits; this contrasts with apparent effects of changing CO2 on plant anatomy over evolutionary time. Future studies should assess how plant responses to drought may be constrained by the apparent shift from tolerance (via low turgor loss point) to avoidance (via stomatal regulation and/or access to deeper soil moisture). For five common species in a semiarid grassland, we assessed the response of several plant hydraulic traits to elevated CO2 (+200 ppm) and warming (+1.5 to 3℃; day to night). Interspecific differences in hydraulic traits were larger than intraspecific shifts induced by elevated CO2 and/or warming, and effects of elevated CO2 were greater than effects of warming. Forbs showed little phenotypic plasticity. Graminoids had leaf water potentials and turgor loss points that were 10 to 50% less negative under elevated CO2; thus, climate change might cause these species to adjust their drought resistance strategy away from tolerance and toward avoidance.