Homozygous deletions on the short arm of chromosome 9 in ovarian adenocarcinoma cell lines and loss of heterozygosity in sporadic tumors

• Published in: American journal of human genetics Vol. 55; no. 1; pp. 143 - 149
• Main Authors: CHENEVIX-TRENCH, G, KERR, J, FRIEDLANDER, M, HURST, T, SANDERSON, B, COGLAN, M, WARD, B, LEARY, J, KHOO, S.-K
• Format: Journal Article
• Language: English
• Published: Chicago, IL University of Chicago Press 01.07.1994
• Subjects: Adenocarcinoma - genetics; Adenocarcinoma, Clear Cell - genetics; Adenocarcinoma, Mucinous - genetics; Adenoma - genetics; BASIC BIOLOGICAL SCIENCES; Biological and medical sciences; Blotting, Southern; BODY; Brenner Tumor - genetics; Carcinoma - genetics; CARCINOMAS; Chi-Square Distribution; CHROMOSOMAL ABERRATIONS; Chromosome Mapping; CHROMOSOMES; Chromosomes, Human, Pair 9; Cystadenocarcinoma, Serous - genetics; DISEASES; DNA, Neoplasm - genetics; DNA, Satellite - genetics; ETIOLOGY; Female; FEMALE GENITALS; Gene Deletion; Genes, Tumor Suppressor; GONADS; Gynecology. Andrology. Obstetrics; Heterozygote; Homozygote; HUMAN CHROMOSOME 9; HUMAN CHROMOSOMES; Humans; Mammary gland diseases; Medical sciences; MUTATIONS; Neoplasm Staging; NEOPLASMS; ORGANS 550400 > Genetics; Ovarian Neoplasms - genetics; OVARIES; Tumor Cells, Cultured; Tumors; Chromosomal aberration; Adenocarcinoma; Human; Heterozygozity; Loss; Malignant tumor; In vitro; Female genital diseases; Homozygozity; Ovarian diseases; Ovary; Cell line; Deletion; Abnormal chromosome; Molecular biology; Southern blotting; Abnormal C9 chromosome
• ISSN: 0002-9297; 1537-6605

Rat ovarian surface epithelial cells transformed spontaneously in vitro have been found to have homozygous deletions of the interferon alpha (IFNA) gene. This suggests that inactivation of a tumor-suppressor gene in this region may be crucial for the development of ovarian cancer. We therefore used microsatellite markers and Southern analysis to examine the homologous region in humans--the short arm of chromosome 9--for deletions in sporadic ovarian adenocarcinomas and ovarian tumor cell lines. Loss of heterozygosity occurred in 34 (37%) of 91 informative sporadic tumors, including some benign, low-malignant-potential and early-stage tumors, suggesting that it is an early event in the development of ovarian adenocarcinoma. Furthermore, homozygous deletions on 9p were found in 2 of 10 independent cell lines. Deletion mapping of the tumors and lines indicates that the candidate suppressor gene inactivated as a consequence lies between D9S171 and the IFNA locus, a region that is also deleted in several other tumors and that contains the melanoma predisposition gene, MLM.