A high-resolution map in the chromosomal region surrounding the Lps locus

• Published in: Genomics (San Diego, Calif.) Vol. 31; no. 3; pp. 283 - 294
• Main Authors: QURESHI, S. T, LARIVIERE, L, SEBASTIANI, G, CLERMONT, S, SKAMENE, E, GROS, P, MALO, D
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier 01.02.1996
• Subjects: Animals; Base Sequence; Biological and medical sciences; Chromosome Mapping; Classical genetics, quantitative genetics, hybrids; DNA Primers; Female; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; Human; Lipopolysaccharides - pharmacology; Male; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; Mice, Inbred DBA; Molecular Sequence Data; Mus spretus; Phenotype; Genetic mapping; High resolution; Linkage; Genetic marker; Rodentia; Chromosome 4; Infection; Resistance; Vertebrata; Sensitivity; Mammalia; Gene; Mouse; Lipopolysaccharide; Genetics; Polymorphism
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1996.0050

The Lps locus on mouse chromosome 4 controls host responsiveness to lipopolysaccharide, a major component of the outer membrane of Gram-negative bacteria. The C3H/HeJ inbred mouse strain is characterized by a mutant Lps allel (Lpsd) that renders it hyporesponsive to LPS and naturally tolerant of its lethal effects. To identify the Lps gene by a positional cloning strategy, we have generated a high-resolution linkage map of the chromosomal region surrounding this locus. We have analyzed a total of 1604 backcross mice from a preexisting interspecific backcross panel of 259 (Mus spretus x C57BL/6J)F1 x C57BL/6J and two novel panels of 597 (DBA/2J x C3H/HeJ)F1 x C3H/HeJ and 748 (C57BL/6J x C3H/HeJ)F1 x C3H/HeJ segregating at Lps. A total of 50 DNA markers have been mapped in a 11.8-cM span overlapping the Lps locus. This positions the Lps locus within a 1.1-cM interval, flanked proximally by a large cluster of markers, including three know genes (Cd301, Hxb, and Ambp), and distally by two microsatellite markers (D4Mit7/D4Mit178). The localization of the Lps locus is several centimorgans proximal to that previously assigned.