Human nicotinamide N-methyltransferase gene : molecular cloning, structural characterization and chromosomal localization

Genomic DNA clones for nicotinamide N-methyltransferase (NNMT), an enzyme that catalyzes drug and xenobiotic metabolism, were isolated from a human chromosome 11-specific DNA library. Study of one of those clones, when combined with PCR-based experiments performed with human genomic DNA, made it pos...

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Published inGenomics (San Diego, Calif.) Vol. 29; no. 3; pp. 555 - 561
Main Authors AKSOY, S, BRANDRIFF, B. F, WARD, A, LITTLE, P. F. R, WEINSHILBOUM, R. M
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier 10.10.1995
Subjects
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Cattle
Chromosome Mapping
Chromosomes, Human, Pair 11
Cloning, Molecular
Codon
DNA Primers
Exons
Fundamental and applied biological sciences. Psychology
Gene Library
Genes. Genome
Humans
In Situ Hybridization, Fluorescence
Introns
Liver - enzymology
Methyltransferases - biosynthesis
Methyltransferases - genetics
Mice
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Nicotinamide N-Methyltransferase
Oligonucleotides, Antisense
Peptide Chain Initiation, Translational
Polymerase Chain Reaction
Rats
Recombinant Proteins - biosynthesis
Regulatory Sequences, Nucleic Acid
Genetic mapping
Human
Nucleotide sequence
Enzyme
Transferases
C11-Chromosome
Homology
Gene organization
Metabolism
Methyltransferases
Chromosome DNA
Transcription initiation
Xenobiotic
Nicotinamide N-methyltransferase
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Summary:Genomic DNA clones for nicotinamide N-methyltransferase (NNMT), an enzyme that catalyzes drug and xenobiotic metabolism, were isolated from a human chromosome 11-specific DNA library. Study of one of those clones, when combined with PCR-based experiments performed with human genomic DNA, made it possible to determine the structure of the human NNMT gene. The gene was approximately 16.5 kb in length and consisted of 3 exons and 2 introns. Transcription initiation for the NNMT gene occurred 105-109 nucleotides 5'-upstream from the cDNA translation initiation codon on the basis of the results of both primer extension and 5'-rapid amplification of cDNA ends. NNMT mapped to chromosome band 11q23.1 by fluorescence in situ hybridization.
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ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1995.9966