Influence of manganese on cell morphology, protoplasts formation and β-d-glucosidase activity in Phaffia rhodozyma

• Published in: FEMS microbiology letters Vol. 171; no. 2; pp. 155 - 160
• Main Authors: Pera, Licia M., Rubinstein, Laura, Baigorı́, Mario D., de Figueroa, Lucía I.C., Callieri, Danley A.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.02.1999; Oxford University Press
• Subjects: Cell morphology; Cell walls; Cytology; Glucosaminidase; Glucosidase; Lytic enzyme; Lytic enzymes; Manganese; Microbiology; Morphology; Phaffia rhodozyma; Potassium chloride; Protoplasts; Protoplasts formation; Yeast; Yeasts; β‐d‐glucosidase; β; glucosidase; Lytic enzyme; Protoplasts formation; Cell morphology; Manganese
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.1999.tb13426.x

Abstract Among several potential cell wall lytic enzymes, such as β-d-glucosidase, β-d-galactosidase, β-N-acetyl-d-glucosaminidase, β-N-acetyl-d-galactosaminidase, and β-d-N,N′-diacetylchitinase, Phaffia rhodozyma showed only β-d-glucosidase activity. MnCl2 increased the activity, but not the production of the enzyme. When the yeast was grown with 4.0 mM MnCl2, the cells were bigger and rounder than those obtained in its absence, suggesting a weakening of the cell wall. Protoplasts were obtained from these cells, using 0.3 M KCl as the osmotic stabiliser.