쌍별귀뚜라미 ethyl acetate 분획의 예쁜꼬마선충에 대한 항산화 효과

Gryllus bimaculatus (GB) is known to have a high protein content and high levels of valine, leucine, and isoleucine. For these reasons, GB is being proposed as a new food resource. A GB methanol extract and fractions resulted in a concentration-dependent increase in the scavenging activity of DPPH,...

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Published in한국식품과학회지 Vol. 56; no. 1; pp. 46 - 54
Main Authors 김현지(Hyeoun-Ji Kim), 문지수(Ji-Su Mun), 강주석(Ju-Seok Kang), 오석흥(Suk-Heung Oh), 김준형(Jun-Hyeong Kim)
Format Journal Article
LanguageKorean
Published 한국식품과학회 01.02.2024
Subjects
식품과학
Caenorhabditis elegans
SOD
antioxidant
Gryllus bimaculatus
ROS
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Summary:Gryllus bimaculatus (GB) is known to have a high protein content and high levels of valine, leucine, and isoleucine. For these reasons, GB is being proposed as a new food resource. A GB methanol extract and fractions resulted in a concentration-dependent increase in the scavenging activity of DPPH, ABTS, and xanthine-derived superoxide radicals. Moreover, the ethyl acetate fraction showed the best scavenging activity. The GB ethyl acetate fraction (GEF) increased superoxide dismutase (SOD) and catalase activities in a dose-dependent manner in Caenorhabditis elegans. This fraction significantly inhibited the production of reactive oxygen species accumulated in the nematodes, and GEF increased the survival rate of nematodes for up to 34 hours under oxidative stress conditions. SOD-3 is a type of SOD, and a green fluorescent protein was attached to it. The expression level of SOD-3::GFP was increased in a dose-dependent manner. Additionally, heat stress resistance increased with an increasing concentration of GEF when culturing C.elegans at 36oC. KCI Citation Count: 2
ISSN:0367-6293
2383-9635
DOI:10.9721/KJFST.2024.56.1.46