들잔디 방사선 변이체의 형태, 생리 및 AFLP 분석을 통한 변이다양성 평가

• Published in: Weed & turfgrass science Vol. 7; no. 4; pp. 387 - 398
• Main Authors: 이긍주(Geung-Joo Lee), 이상기(Sang Ki Lee), 김영선(Young-Sun Kim), 전용삼(Yongsam Jeon), 이가연(Ka Youn Lee)
• Format: Journal Article
• Language: Korean
• Published: 한국잡초학회·한국잔디학회 01.12.2018; 한국잔디학회
• Subjects: 농학; Gamma ray; Proton beam; Polymorphic information content; Zoysiagrass; PCR-based AFLP
• ISSN: 2287-7924; 2288-3312
• DOI: 10.5660/WTS.2018.7.4.387

With limited genetic resources, traditional methods of turfgrass breeding such as plant introduction, recurrent selection, and hybridization have shown difficulties of genetic recombination and increase in genetic variation. Considering the challenges, radiationinduced mutation is an alternative tool to extend genetic diversity in the short term. This study was to assess morphological and genetic difference of radiation-induced Korean lawngrass ( Z. japonica ) mutants from non-radiated wild type. Those mutants irradiated with 200-250 Gy radiation were selected for their unique morphological and physiological characteristics including fine-leaf, dwarf, higher tiller and density, or low temperature greening. Compared to pot evaluation under green house, all the morphological traits were significantly different among accessions in the field conditions. Selected mutants showing reduced leaf width, angle, and plant height demonstrated consistent performance both in pot and field studies. Selections exhibiting green color retention in the beginning of dormancy were found to have lower malondialdehyde (MDA) content, indicating resistant to oxidation of lipid membrane with lower temperature. The phylogenic tree from the amplified fragment length polymorphism (AFLP) analysis verified the expanded genetic diversity, where nonirradiated control was grouped in a separate clade, with farther genetic distance from other mutants. The polymorphic information content describing the chance of different allele at marker loci indicates that about 16% of the allele changes was partly attributed to gamma radiation or proton beam as a physical mutagen. The confirmed results demonstrated that the mutation induction can secure desirable traits of zoysiagrass, with providing the enlarged genetic diversity. KCI Citation Count: 0