추출용매에 따른 당삼 추출물의 미백 활성 변화

• Published in: KSBB Journal Vol. 34; no. 1; pp. 1 - 9
• Main Authors: 박현지(Hyun Ji Park), 안소희(So Hee Ahn), 최원영(Won Yeoung Choi), 이계원(Gye Won Lee), 권미진(Mi Jin Kwon), 조영호(Young Ho Cho)
• Format: Journal Article
• Language: Korean
• Published: 한국생물공학회 01.03.2019
• Subjects: 생물공학; melan-a cells; Codonopsis pilosula; anti-melanogenic effect; anti-oxidant activity
• ISSN: 1225-7117; 2288-8268
• DOI: 10.7841/ksbbj.2019.34.1.1

This study was designed to compare the anti-oxidant activity and anti-melanogenic effect of Codonopsis pilosula extracts (CPE) according to extraction solvents. Codonopsis pilosula was treated with distilled water (CPE-DW) and 70% EtOH (CPE-EtOH), respectively. The total polyphenol and flavonoid contents of CPE-EtOH and CPE-DW were 6.11, 8.54 μg/mg and 1.59, 2.44 μg/mg, respectively. In antioxidant activities through DPPH, ABTS radical scavenging activity, and reducing power of CPE, CPE-DW showed higher scavenging activities than those of CPE-EtOH. Furthermore, The melanin contents of CPE-EtOH and DW treatment at 100 μg/mL were significantly inhibited by 22% and 33% in melana cells, respectively (p<0.01). Its anti-melanogenic effect was originated from the inhibition of intra-cellular tyrosinase activity and melanogenesis related protein expression by CPE treatment in a dose dependent manner. Taken together, We suggest that Codonopsis pilosula extracts may be useful as a natural active ingredient for whitening cosmetics. KCI Citation Count: 3