들깨박으로부터 항산화 활성 물질의 동정

• Published in: Han'guk Sikp'um Yŏngyang Kwahakhoe chi Vol. 49; no. 8; pp. 900 - 906
• Main Authors: 정경한(Gyeong Han Jeong), 정윤희(Yun Hee Jeong), 남정호(Jung Ho Nam), 김태훈(Tae Hoon Kim)
• Format: Journal Article
• Language: Korean
• Published: 한국식품영양과학회 01.08.2020
• Subjects: 식품과학; radical scavenging; DPPH; defatted Perilla cake; ABTS?; phenolic constituent
• ISSN: 1226-3311; 2288-5978
• DOI: 10.3746/jkfn.2020.49.8.900

들깨박의 열수 추출물의 EtOAc 가용부는 DPPH 및 ABTS+ 라디칼을 활용한 라디칼 소거능 평가에서 IC50값이 각각 167.5±1.8, 28.0±1.1 μg/mL로 우수한 라디칼 소거 활성을 나타냄을 확인하였다. 활성성분 동정을 위하여 EtOAc 분획물에 대해 반복적인 컬럼크로마토그래피를 활용하여 활성 물질의 분리 및 정제를 수행하여 10종의 페놀성 화합물을 분리하였고, 각 화합물의 구조는 NMR, FABMS 스펙트럼 해석 및 표품과의 HPLC 직접 비교를 통하여 rosmarinic acid 3-O-glucoside(1), rosamrinic acid(2), caffeic acid (3), p-coumaric acid(4), vanillic acid(5), vanillin(6), protocatechuic acid(7), protocatechuic aldehyde(8), p- hydroxybenzoic acid(9) 및 p-hydroxybenzaldehyde(10)로 동정하였다. 분리된 화합물에 대해 라디칼 소거 활성을 평가한 결과, romarinic acid(2)가 DPPH 및 ABTS+ 라디칼에 대해 가장 우수한 라디칼 소거 활성(IC50＝34.1±0.8, 20.6±1.0 μM)을 나타냄을 확인하였으며, 또한 caffeic acid (3)는 DPPH 및 ABTS+ 라디칼에 대해 IC50값이 각각 82.7 ±1.8, 41.4±1.6 μM의 라디칼 소거능을 확인하였다. 들기름 착유 후의 들깨박에는 우수한 라디칼 소거능을 나타내는 다량의 페놀성 화합물이 함유되어 있는 것을 확인하였으며, 향후 세포 및 동물을 활용한 연구를 통하여 기능성 소재로서의 추가 검증이 필요하다고 생각된다. The antioxidant capacity of the hot water extract of defatted Perilla cake were evaluated using DPPH and ABTS+ radical scavenging assays. The extracts of four solvent fractions (n-hexane layer, EtOAc layer, n-BuOH layer, and residue) of the hot water extract were also investigated. In particular, the radical scavenging activity of the EtOAc-soluble portion from defatted Perilla cake was higher than those of the other solvent-soluble layers. This paper reports the chromatographic isolation and characterization of ten phenolic constituents of previously known structures, rosmarinic acid 3-O-glucoside (1), rosmarinic acid (2), caffeic acid (3), p-coumaric acid (4), vanillic acid (5), vanillin (6), protocatechuic acid (7), protocatechuic aldehyde (8), p-hydroxybenzoic acid (9), and p-hydroxybenzaldehyde (10). The chemical structures of these isolated compounds were identified by neclear magnetic resonance (NMR) spectroscopy and mass spectrometry methods. Among the compounds tested, rosmarinic acid (2) and protocatechuic aldehyde (8) showed the most potent DPPH radical scavenging activities with IC50 values of 34.1±0.8 and 74.2±1.2 μM, respectively. In addition, these two compounds showed significant ABTS+ radical scavenging capacities. These results suggest that the radical scavenging activities of defatted Perilla cake are closely correlated with the presence of phenolic compounds. KCI Citation Count: 7