Bacillus licheniformis TK3-Y를 이용한 150 L 반응기에서의 고염분 함유 수산폐기물 생분해 및 분해 산물의 액비 이용 가능성 평가

• Published in: KSBB Journal Vol. 35; no. 4; pp. 288 - 293
• Main Authors: 강경환(Kyeong Hwan Kang), 이희진(Heejin Lee), 박태진(Taejin Park), 심지한(Ji Han Sim), 김승영(Seung-Young Kim)
• Format: Journal Article
• Language: Korean
• Published: 한국생물공학회 01.12.2020
• Subjects: 생물공학; reutilization; Bacillus licheniformis TK3-Y; biodegradation; high salinity fishery waste; liquid fertilizer
• ISSN: 1225-7117; 2288-8268
• DOI: 10.7841/ksbbj.2020.35.4.288

High salinity fishery wastes (HFW) containing seaweed and fish waste cause great influence on the local environment. However, the fishery wastes contain biodegradable components such as carbohydrates, proteins and lipids that are able to be converted to useful bioactive substances by useful bacterium. Therefore, we evaluated large scale biodegradation property of 17.5% of salinity HWF containing green seaweed and anchovy waste by Bacillus licheniformis TK3-Y in 150-L fermenter and utilization of HFW biodegraded product as liquid fertilizer. During 7-day biodegradation, the pH increased with increasing cell density. The decrease in DO (dissolved oxygen) and ORP (oxidation-reduction potential) meant that the HFW was actively biodegraded. CODcr (chemical oxygen demand) and T-N (total nitrogen) were also removed by 87.3 and 87.1%, respectively. In addtion, B.licheniformis TK3-Y showed 0.45 U/mL cellulolytic, 1,016 U/mL proteolytic, and 5.92 U/mL lipolytic activities. The 500- and 1000- fold diluted supernatant from the culture of HFW had no phytotoxicity. In barley hydroponic culture, the 1,000-fold diluted culture supernatant showed a good fertilizing ability. This study indicates the large-scale treatment of HFW and the biodegraded products are sufficient for agricultural value. KCI Citation Count: 0