일회성 장시간의 유산소 운동 수행에 의한 노화쥐의 조직별 지방산화 관련 단백질의 발현 변화

• Published in: 한국체육과학회지 Vol. 21; no. 2; pp. 939 - 948
• Main Authors: 변후균(Byun, Hoo-Kyoun), 여효성(Yeo, Hyo-Seong), 어수주(Eo, Su-Ju), 김창근(Kim, Chang-Keun), 김효정(Kim, Hyo-Jeong)
• Format: Journal Article
• Language: Korean
• Published: 한국체육과학회 01.04.2012
• Subjects: 체육; HSL; swimming; LPL; aging
• ISSN: 1226-0258; 3022-487X

Aging has been considered to be a crucial risk factor for metabolic disoder. Lipoprotein lipase (LPL) is the enzyme responsible for the hydrolysis of triglyceride (TG)-rich lipoproteins and plays an important role in directing free fatty acids toward adipose and muscle tissues. Hormone-sensitive lipase (HSL) plays a crucial role in the hydrolysis of triacylglycerol in various tissues as well. The purpose of this study was to examine the effect of an acute long endurance exercise on LPL and HSL expression to tissue type in old rat. 78-week-old female rats(n=16) were used and divided into two groups, control (CON, n=8) and exercise group(EXE, n=8). All animals in the exercise group performed one bout of 3-hr swimming (30min x 6 bouts with 5min rest). We measured serum glucose, insulin concentration and total glycogen content. Protein analyses were performed with gastrocnemius red vs. white and visceral vs. subcutaneous fat tissue using western-blotting. All data are presented as means±SD. Independent samples t-test was used to determine changes in groups. Differences were considered significant if p<.05. Following the long endurance swimming exercise, the HSL and LPL protein expression were decreased by 33% and 39% in gastrocnemius red muscle(p<.05). In addition, HSL and LPL protein expression were increased by 54% and 76%, respectively, in subcutaneous fat(SF, p<.05). These results suggest the possibility of metabolic disorder in aged skeletal muscle. KCI Citation Count: 1