淸燥救肺湯의 Nrf2 매개 항산화 효능

• Published in: 동의생리병리학회지 Vol. 29; no. 2; pp. 174 - 179
• Main Authors: 이광규(Kwang Gyu Lee), 이학인(Hak In Lee), 정한솔(Han-Sol Jeong)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 01.04.2015
• Subjects: 한의학; Cheongjogupye-tang; Nrf2; Anti-oxidant response element
• ISSN: 1738-7698; 2288-2529
• DOI: 10.15188/kjopp.2015.04.29.2.174

Transcription factor, Nrf2 was well known to protect cell from oxidative stress by up-regulating it’s dependent anti-oxidative genes such as HO-1 and NQO1. Cheongjogupye-tang (CJGPT), a traditional herbal formula was originally recorded in 『EuiMunBeopRyul』, still having been used to treat pulmonary disease such as asthma and pulmonary inflammation, in Eastern Asian countries. However, the underlying therapeutic mechanisms remain elusive. The purpose of this study is to investigate the anti-inflammatory or anti-oxidative effects of CJGPT on the RAW 264.7 cells. To examine the anti-inflammatory or anti-oxidative effects of CJGPT, MTT assay, immunoblotting, RT-PCR and reporter gene assays were performed. Although CJGPT slightly suppressed the nuclear NF-κB expression, it did not decreased the expression of pro-inflammatory genes in LPS-stimulated RAW 264.7 cells. Moreover, it did not increased the transcriptional activity of NF-κB in reporter gene assay. However, CJGPT upregulated the nuclear expression of Nrf2, as well as increased the expression of Nrf2-dependent genes such as HO-1 and NQO1. In addition, CJGPT incresed the transcriptional activity of Nrf2. Taken together, our results showed that CJGPT exerts functions as an anti-oxidant mainly by activating Nrf2. KCI Citation Count: 1