桑椹子추출물의 Peroxynitrite 억제 및 염증 촉진 인자 제어 효과

• Published in: 동의생리병리학회지 Vol. 22; no. 6; pp. 1475 - 1481
• Main Authors: 우성호(Sung Ho Woo), 정지천(Ji Cheon Jeong), 신현철(Hyeon Cheol Shin)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 01.12.2008
• Subjects: 한의학
• ISSN: 1738-7698; 2288-2529

This study was to investigate the inhibitory effects of Mori Fructus on the generation of peroxynitrite (ONOO-), nitric oxide (NO) and superoxide anion radical (․O2-) in the endothelial cells of rat vessels. The aim of this study was to investigate the ONOO-, NO, ․O2- scavenging and anti-inflammatory activitives of Mori Fructus. For this study, the fluorescent probes, namely dihydrorhodamine 123 (DHR 123), 4,5-diaminofluorescein (DAF-2) and 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) were used. Western blotting was performed using anti-NF-κB (p50, p65), anti-COX-2, anti-iNOS antibodies, respectively. Mori Fructus prevented lipopolysaccharide (LPS)-induced cell death in YPEN cells. Mori Fructus inhibited the generation of ONOO-, NO and ․O2- in the LPS-treated cells. Mori Fructus inhibited the expression of COX-2 and iNOS genes by means of decreasing the NF-κB activation. These results suggest Mori Fructus is effective on inhibiting the generation of ONOO-, NO and ․O2-, and that therefore it might have a potential role as a treatment for the inflammatory process and inflammation-related diseases. KCI Citation Count: 1