太陰調胃湯加減方의 db/db 마우스 肝에 대한 아디포사이토카인 및 SREBPs의 발현에 미치는 영향

• Published in: 동의생리병리학회지 Vol. 24; no. 6; pp. 989 - 995
• Main Authors: 백종우(Jong Woo Baek), 정미경(Mi Kyung Jeong), 고성규(Seong Gyu Ko), 전찬용(Chan Yong Jun), 박종형(Jong Hyeong Park), 최유경(Kyung Choi)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 01.12.2010
• Subjects: 한의학; TNF-α; SREPBs; Taeeumjowuitanggagam-bang(TJV); IL-6; obesity
• ISSN: 1738-7698; 2288-2529

The aim of this study was to investigate the effect of Taeeumjowuitanggagam-bang(TJV) on the mRNA expression of adipocytokines (TNF-α and IL-6), and SREBPs. Mice were divided into 4 groups ; a normal group of db/+ mice, a control group of db/db mice, a group (db/db mice) treated with TJV 200 mg/kg, and a group (db/db mouse) treated with TJV 500 mg/kg. They were treated orally with TJV and measured their body weight every other day during 9 weeks. After that, we measured the mRNA expression of adipocytokines (TNF-α and IL-6) and SREBPs (SREBE-1a and SREBP-1c) in liver, and blood concentration of total cholesterol, triglyceride, too. In addition, liver samples were fixed in 4% PFA for 2 hours and stored in -70℃. Liver tissues were embedded in Optical cutting temperature(OCT) compound and 30 ㎛ sections were cut. Tissue sections were stained Oil-Red-O to visualize neutral lipids. Nuclei were stained with hematoxylin solution. In result, the TJV reduced the mRNA expressions of TNF-α and IL-6 and SREBPs in liver and stained liver tissue less red than control group. However, there was no significant differences in total cholestreol and triglyceride blood concentration and body weight among groups. The TJV has inhibitory effect on the mRNA expression of adipocytokines and SREBPs. Therefore, it is assumed that the TJV is related to inhibiting lipogenesis in the liver. KCI Citation Count: 0