아토피피부염 동물 병태 모델에서 石紫解肌湯의 면역조절작용에 관한 연구

• Published in: 동의생리병리학회지 Vol. 26; no. 4; pp. 446 - 454
• Main Authors: 이병웅(Byung Woong Lee), 김선빈(Seon Bin Gim), 송향희(Hyang Hee Song), 지중구(Joong-Gu Ji), 박지원(Ji won Bak), 김동희(Dong Hee Kim)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 01.08.2012
• Subjects: 한의학; Clinical skin index; Animal model; Cytokines; Atopic dermatitis(AD); Seokjagaeki-tang; Immune cell
• ISSN: 1738-7698; 2288-2529

In order to evaluate the efficacy of SHT against atopic dermatitis (AD), various immune related cytokines as well as histological comparison were performed in animal models, and the results are described. Clinical skin index of the SHT treated group decreased significantly in weeks 11 and 13, compared to the control group. Also, CD4+ immune cell ratio in the dorsal skin was significantly decreased to 69%, and both epidermal and dermal skin thickness was decreased. Serum IL-4, IL-5, IL-6, IL-13, and TNF-α, which are all important markers of inflammation, were decreased to 64%, 44%, 87%, 48%, and 45%, respectively. The expression of histamine, a chemical transmitter increasingly released during the progression of inflammation, was significantly decreased to 47%. The production of IgE immunoglobulin was significantly decreased to 16% compared to the control group. In conclusion, SHT pacifies the activation of T cells, leading to suppression of both Th2 cytokine overexpression and infiltration of immune cells into skin. As a result, relative thinning of both epidermis and dermis were observed. With the results obtained from in vitro studies, the immune modulatory effect of SHT in AD animal models was experimentally demonstrated. This study should provide solid information to construct EBM and for clinical practice. KCI Citation Count: 3