The Modulation of Cell Wall-Associated Triton X-100 Soluble Protein (TSP) Antigens of Mycobacterium tuberculosis on Murine Dendritic Cells

• Published in: Laboratory animal research Vol. 24; no. 4; pp. 659 - 667
• Main Authors: Yung-Choon Yoo, Young-Joo Bae, Tae-Hyun Paik, Junglim Lee
• Format: Journal Article
• Language: Korean
• Published: 한국실험동물학회 01.12.2008
• Subjects: 수의학; murine dendritic cells; Mycobacterium tuberculosis; IL-6; TSP antigens
• ISSN: 1738-6055; 2233-7660

Mycobacterium tuberculosis or its secreted protein antigens are known as a potent inducer of maturation process of dendritic cells, during which the expression of cell surface molecules and the capability of antigen presentation are increased, resulting in potent interaction with T cells to initiate the acquired immune response against tuberculosis. In this study, the cell wall-associated Triton X-100 soluble protein (TSP) antigens, TSP- BCG, TSP-H37Ra, TSP- H37Rv and TSP-K, were tested on murine bone marrowderived dendritic cells to determine the capability of their immunologic modulation. Only TSP-H37Rv stimulated murine dendritic cells showed moderate production of IL-6 and MIP-1α compared to those of LPS. TSP-H37Rv antigen did not induce the production of IL-12, TNF-α or IL-10. Other TSP antigenstimulated murine dendritic cells showed minimal or no induction of cytokines (IL-6, IL-12, TNF-α or IL- 10) or chemokines (MIP-1α or RANTES). The murine dendritic cells showed the production of NO as well as the expression of iNOS mRNA after LPS stimulation, however, none of TSP antigen-stimulated murine dendritic cells induced either NO or iNOS mRNA expression. The allostimulation of T cells by murine dendritic cells, which were stimulated with each TSP antigens, showed insignificant increase in T cell proliferation activity in mixed lymphocyte reaction. These results suggested that cell wall-associated TSP antigen isolated from Mycobacterium tuberculosis H37Rv strain acts as a more potent stimulator on murine dendritic cells than any other TSP antigens, even the stimulation from TSP-H37Rv was not enough to activate the maturation of process of murine dendritic cells. KCI Citation Count: 0