Cisplatin 유도 급성신부전에서 Klotho 단백질의 발현

• Published in: Korean journal of veterinary research Vol. 54; no. 4; pp. 225 - 231
• Main Authors: 박소라(So Ra Park), 김태원(Tae Won Kim), 김영중(Young Jung Kim), 김현태(Hyun Tae Kim), 류시윤(Si Yun Ryu), 정주영(Ju Young Jung)
• Format: Journal Article
• Language: Korean
• Published: 대한수의학회 01.12.2014
• Subjects: 수의학; localization; inflammatory cytokine; acute kidney injury; Klotho
• ISSN: 2466-1384; 2466-1392
• DOI: 10.14405/kjvr.2014.54.4.225

Klotho deficiency is an early event in acute kidney injury (AKI) that exacerbates acute kidney damage. The present study explored the expression of Klotho and inflammation related factors in cisplatin-induced AKI. Rats(n = 18) were treated with cisplatin intraperitoneal injection (5 mg/kg) or left untreated as controls (n = 6), then sacrificedat 5 (n = 6) and 10 days (n = 6) treatment. Five days after cisplatin injection, the serum kidney enzymes and kidneycell apoptosis were significantly increased. Moreover, the expression of Klotho was decreased when compared to thecontrol group, especially in the cortex and outer medulla regions. In contrast, inflammation related signals includingnuclear factor kappa B, tumor necrosis factor-α, and tumor necrosis factor-like weak inducer of apoptosis were enhanced. However, 10 days after cisplatin injection, Klotho expression was enhanced upon both IHC and Western blot analysis,with slightly recovered renal function and decreased apoptosis. Furthermore, inflammation related signals expressionwas decreased relative to the 5 days group. Overall, this study confirmed the opposite expression patterns betweenKlotho and inflammation related signals and their localization in cisplatin-induced AKI kidney. KCI Citation Count: 0