베체트병의 동물모형에 대한 瀉心湯類 투여 효과에 관한 연구

• Published in: 동의생리병리학회지 Vol. 18; no. 4; pp. 1061 - 1070
• Main Authors: 이선구(Seon Goo Lee), 안규석(Kyoo Seok Ahn)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 01.08.2004
• Subjects: 한의학
• ISSN: 1738-7698; 2288-2529

Chronic oral aphthae, recurrent ulcer and uveitis are the three main festations of Behҫet´s disease(BD). The aetiopathogenesis of Behҫet´s disease is still obscure, but herpes simplex virus is one of the possible casual factors. Gamchosasim-tang(Gancaoxiexin-tang), Banhasasim-tang(Banxiaxiexin-tang) and Saenggangsasim-tang( Shengjiangxiexin-tang) are traditional medication in Oriental medicine, that has been used to treat inflammatory disease. Especially, Gamchosasim-tang used to treat Behҫet´s disease like symptoms. ICR mice were used for this study. The earlobe of the mice were scratched with a needle, then inoculation with 1.0 X 106 plaque forming units/ml of HSV type Ⅰ. Virus inoculation was performed twice with 10 day interval, followed by 16 weeks fo observation. Using the HSV-induced Behҫet´s disease mouse model, kinds of Sasim-tang were administered variously before and after inoculation. In order to classify the symptomatic mice as having Behҫet´s disease like symptoms. We followed the revised Japanese classification with minor modifications. Ulceration of the mice were monitored. In addition, spleen cytokine expression were measured by polymerase chain reaction, ELISA. HSV DNA was detected in HSV inoculation mice. HSV-induced mice treated with kinds of Sasim-tang showed improvement in symptom. In RT-PCR results, IFN-γ was expressed for all groups, IL-2 was expressed for the treated groups, and IL-10 was also expressed. IL-4 was expressed nothing. In ELISA, IL-2 was increased for GSST 2, BSST 2, GSST 2, GSST3 and INF-γ was increased for GSST 2, BSST 2, SSST 2, SSST 3. This model suggest the possible role of immmune response to viral infection in the development and activation of Behҫet´s disease. KCI Citation Count: 1