蔓蔘의 과산화수소에 의한 SK-N-MC의 세포사에 미치는 영향

• Published in: 동의생리병리학회지 Vol. 22; no. 2; pp. 328 - 332
• Main Author: 이선구(Seon Goo Lee)
• Format: Journal Article
• Language: Korean
• Published: 한의병리학회 01.04.2008
• Subjects: 한의학
• ISSN: 1738-7698; 2288-2529

The purpose of this study was to identify the protective effect of Codonopis pilosula extract on cell death induced by H2O2 in SK-N-MC neuroblastoma cells. We measured the antioxidant effect by DPPH radical scavenging analysis, BSA analyssis and examined the cell viability by crystal violet and cytochrome C, Bax, Bcl-2, p53, p21 by using Western blot analysis. Codonopis pilosula extract scavenged DPPH radical in a dose-dependent manner and shown direct free radical scavenging effect, suggested that Codonopis pilosula extract have antioxidant effect in vitro. Treatment of cells with hydrogen peroxide, a reactive oxygen species, was to induce cell death and pretreatment with Codonopis pilosula extract attenuated the occurrence of H2O2-induced cell death. To elucidate the protective mechanisms of action of Codonopis pilosula extract, Western blot analyses for Bcl-2 and Bax expression and cytochrome c release were carried out. Pretreatment with Codonopis pilosula extract induced the expression of Bcl-2 and suppressed the release of cytochrome c and Bax into the cytosol, thereby arresting H2O2-induced apoptotic cell death. Especially p21 and p53 were decreased prior to H2O2 treatment. These results suggest that Codonopis pilosula extract is associated with the cell cycle and anti-apoptotic cell death. KCI Citation Count: 1