위암 발생의 민감성과 RNase3 유전자 다형성과의 연관성

• Published in: Annals of surgical treatment and research Vol. 78; no. 5; pp. 283 - 289
• Main Authors: 구자욱(Ja Wook Koo), 강동백(Dong Baek Kang), 박원철(Won Cheol Park), 이영환(Young Hwan Lee), 강인홍(In Hong Kang), 채수천(Soo Cheon Chae), 이정균(Jeong Kyun Lee)
• Format: Journal Article
• Language: Korean
• Published: 대한외과학회 01.05.2010
• Subjects: 일반외과학; 위암; RNase3; Gastric cancer; 유전자 다형성; Polymorphism
• ISSN: 2288-6575; 2288-6796

Purpose: RNase3 is a secretory ribonuclease, which is found in the eosinophilic leukocyte and involved in the innate immune system. Its cytotoxic activity is effective against a wide range of pathogens. We performed a case-control study to examine the relationship between RNase3 polymorphisms and the susceptibility of gastric cancer in Korean people. Methods: Blood sampling of stomach cancer and healthy persons groups were performed, Taqman in g.-550A＞G, polymerase chain reaction-restriction fragment length polymorphism in g.371C＞G, and high-resolution melt in g.499C＞G were analyzed. The three single nucleotide polymorphisms g.-550A＞G, g.371C＞G, and g.499C＞G in RNase3 and their haplotypes were analyzed. Results: The genotype and allele frequencies of RNase3 g.-550A＞G and g.371C＞G were not significantly increased in susceptibility of gastric cancer than control group. But, RNase3 CC genotype was associated with a significantly increased susceptibility of gastric cancer than control group (P=0.002). Also, RNase3 CC genotype was more specifically associated with a significantly increased susceptibility of middle and lower gastric cancer than upper gastric cancer (P=0.002). In haplotype of RNase3 SNP g.-550A, g.371G, and g.499C, there was significantly susceptibility of gastric cancer (P=0.004), and more specific influence on middle and lower gastric cancer than upper gastric cancer (P=0.006 vs 0.054). Conclusion: RNase3 g.499C＞G polymorphism may influence gastric cancers, and have a more specific influence on middle and lower gastric cancer rather than upper gastric cancer. But RNase3 g.-550A＞G, g.371C＞G polymorphisms need careful interpretation and confirmation in more larger studies. Purpose: RNase3 is a secretory ribonuclease, which is found in the eosinophilic leukocyte and involved in the innate immune system. Its cytotoxic activity is effective against a wide range of pathogens. We performed a case-control study to examine the relationship between RNase3 polymorphisms and the susceptibility of gastric cancer in Korean people. Methods: Blood sampling of stomach cancer and healthy persons groups were performed, Taqman in g.-550A＞G, polymerase chain reaction-restriction fragment length polymorphism in g.371C＞G, and high-resolution melt in g.499C＞G were analyzed. The three single nucleotide polymorphisms g.-550A＞G, g.371C＞G, and g.499C＞G in RNase3 and their haplotypes were analyzed. Results: The genotype and allele frequencies of RNase3 g.-550A＞G and g.371C＞G were not significantly increased in susceptibility of gastric cancer than control group. But, RNase3 CC genotype was associated with a significantly increased susceptibility of gastric cancer than control group (P=0.002). Also, RNase3 CC genotype was more specifically associated with a significantly increased susceptibility of middle and lower gastric cancer than upper gastric cancer (P=0.002). In haplotype of RNase3 SNP g.-550A, g.371G, and g.499C, there was significantly susceptibility of gastric cancer (P=0.004), and more specific influence on middle and lower gastric cancer than upper gastric cancer (P=0.006 vs 0.054). Conclusion: RNase3 g.499C＞G polymorphism may influence gastric cancers, and have a more specific influence on middle and lower gastric cancer rather than upper gastric cancer. But RNase3 g.-550A＞G, g.371C＞G polymorphisms need careful interpretation and confirmation in more larger studies. KCI Citation Count: 0