Collagen-derived dipeptide prolyl-hydroxyproline promotes differentiation of MC3T3-E1 osteoblastic cells

• Published in: Biochemical and biophysical research communications Vol. 453; no. 3; pp. 498 - 501
• Main Authors: Kimira, Yoshifumi, Ogura, Kana, Taniuchi, Yuri, Kataoka, Aya, Inoue, Naoki, Sugihara, Fumihito, Nakatani, Sachie, Shimizu, Jun, Wada, Masahiro, Mano, Hiroshi
• Format: Journal Article
• Language: English
• Published: United States 24.10.2014
• Subjects: 3T3 Cells; 60 APPLIED LIFE SCIENCES; ALKALINE PHOSPHATASE; Alkaline Phosphatase - metabolism; AMP; Animals; Calcification, Physiologic; Cell Differentiation - physiology; CELL PROLIFERATION; COLLAGEN; Collagen - metabolism; Collagen Type I - metabolism; CONNECTIVE TISSUE CELLS; Core Binding Factor Alpha 1 Subunit - genetics; Dipeptides - metabolism; Dipeptides - physiology; GENES; HYDROXYPROLINE; Mice; MINERALIZATION; Osteoblasts - cytology; PEPTIDES; PROPANOLS; Real-Time Polymerase Chain Reaction; Sp7 Transcription Factor; Transcription Factors - genetics; Collagen-derived peptide; Prolyl-hydroxyproline; Osteoblast differentiation
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2014.09.121

Prolyl-hydroxyproline (Pro-Hyp) is one of the major constituents of collagen-derived dipeptides. The objective of this study was to investigate the effects of Pro-Hyp on the proliferation and differentiation of MC3T3-E1 osteoblastic cells. Addition of Pro-Hyp did not affect MC3T3-E1 cell proliferation and matrix mineralization but alkaline phosphatase activity was significantly increased. Furthermore, cells treated with Pro-Hyp significantly upregulated gene expression of Runx2, Osterix, and Col1α1. These results indicate that Pro-Hyp promotes osteoblast differentiation. This study demonstrates for the first time that Pro-Hyp has a positive effect on osteoblast differentiation with upregulation of Runx2, Osterix, and Collα1 gene expression.