Prostaglandin E2 catabolism in the spinal nerve roots of the cat

Catabolism of prostaglandin (PG) E2 was studied in homogenates of spinal cord and spinal nerve roots of the cat. Spinal roots enzymatically converted PGE2 to a product (metabolite I) with the chromatographic mobility of 15-keto-PGE2. Little metabolic degradation occurred in the spinal cord; however,...

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Bibliographic Details
Published inCanadian journal of physiology and pharmacology Vol. 58; no. 2; p. 227
Main Authors Bishai, I, Coceani, F
Format Journal Article
LanguageEnglish
Published Canada 01.02.1980
Subjects
Animals
Cats - metabolism
Female
Hydroxyprostaglandin Dehydrogenases - metabolism
In Vitro Techniques
Male
Prostaglandins E - metabolism
Spinal Cord - metabolism
Spinal Nerve Roots - metabolism
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Summary:Catabolism of prostaglandin (PG) E2 was studied in homogenates of spinal cord and spinal nerve roots of the cat. Spinal roots enzymatically converted PGE2 to a product (metabolite I) with the chromatographic mobility of 15-keto-PGE2. Little metabolic degradation occurred in the spinal cord; however, incubation of PGE2 with combined spinal cord and nerve root tissue yielded a second metabolite (metabolite II) in addition to metabolite I. Metabolite II was identified as 15-keto-13, 14-dihydro-PGE2. These results prove that spinal nerve roots, unlike the spinal cord, contain 15-hydroxyprostaglandin dehydrogenase (15-PGDH) which is the major and rate-limiting enzyme in the inactivation of prostaglandins. The location and functional significance of 15-PGDH in peripheral nerves remain to be elucidated.
ISSN:0008-4212
DOI:10.1139/y80-039