Microfluidic designs and techniques using lab-on-a-chip devices for pathogen detection for point-of-care diagnostics
Effective pathogen detection is an essential prerequisite for the prevention and treatment of infectious diseases. Despite recent advances in biosensors, infectious diseases remain a major cause of illnesses and mortality throughout the world. For instance in developing countries, infectious disease...
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| Published in | Lab on a chip Vol. 12; no. 18; pp. 3249 - 3266 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
21.09.2012
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| Subjects | |
| Online Access | Get full text |
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| Summary: | Effective pathogen detection is an essential prerequisite for the prevention and treatment of infectious diseases. Despite recent advances in biosensors, infectious diseases remain a major cause of illnesses and mortality throughout the world. For instance in developing countries, infectious diseases account for over half of the mortality rate. Pathogen detection platforms provide a fundamental tool in different fields including clinical diagnostics, pathology, drug discovery, clinical research, disease outbreaks, and food safety. Microfluidic lab-on-a-chip (LOC) devices offer many advantages for pathogen detection such as miniaturization, small sample volume, portability, rapid detection time and point-of-care diagnosis. This review paper outlines recent microfluidic based devices and LOC design strategies for pathogen detection with the main focus on the integration of different techniques that led to the development of sample-to-result devices. Several examples of recently developed devices are presented along with respective advantages and limitations of each design. Progresses made in biomarkers, sample preparation, amplification and fluid handling techniques using microfluidic platforms are also covered and strategies for multiplexing and high-throughput analysis, as well as point-of-care diagnosis, are discussed. NRC publication: Yes |
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| Bibliography: | Tohid Fatanat Didar is a PhD student in the Department of Biomedical Engineering at McGill University. He received his bachelors degree in mechanical engineering from Sharif University of Technology. During his master's studies, he worked on micro/nano fabrication of microfluidic platforms. His current research involves implementing micro and nanofabrication principles to produce micro-chips for biological applications. He designs microfluidic platforms with bio-functional interfaces to specifically detect, separate or investigate biological elements. Maryam Tabrizian is full professor at the Department of Biomedical Engineering at McGill University. She is FRSQ-Chercheur National awardee and became the Guggenheim Fellow in Biomedical Sciences in 2010. M. Tabrizian's core competency is in the field of biointerface, biorecognition systems and microfluidics and their integration with biosensing devices for cell behaviour investigation in stimulated microenvironments. For more than 10 years, her laboratories have been working on the development of microfluidic platforms compatible with impedance spectroscopy and surface plasmon resonance spectroscopy for the detection and high throughput analysis of biomarkers, proteins, peptides, DNA, drugs and other biologically active substances. Amir M. Foudeh is a PhD student in the Department of Biomedical Engineering at McGill University. He received his bachelor's degree in Chemical Engineering from Isfahan University of Technology, Iran and obtained his master's degree in Biotechnology from Chalmers University of Technology, Goteborg, Sweden. His primary research is focused on developing microfluidic-based biosensors for the detection of pathogens. Teodor Veres is a Senior Research Officer and the group leader of the Functional Nanomaterials Group in the Life Sciences Division of the National Research Council of Canada. He is also adjunct professor in the Department of Biomedical Engineering at McGill University. In NRC, Dr Veres is leading the activities related to the design, fabrication and use of microfluidic components and systems for diagnostics applications as well as the synthesis and characterization of functional nanostructures for applications in drug delivery, bio-sensing isolation of molecular of biological targets in microfluidic systems. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 ObjectType-Review-3 content type line 23 |
| ISSN: | 1473-0189 1473-0197 1473-0189 |
| DOI: | 10.1039/c2lc40630f |