Genetic and Functional Analyses of the DKxanthene Biosynthetic Gene Cluster from Myxococcus stipitatus DSM 14675

DKxanthenes are a class of yellow secondary metabolites produced by myxobacterial genera and . We identified a putative 49.5 kb DKxanthene biosynthetic gene cluster from DSM 14675 by genomic sequence and mutational analysis. The cluster was comprisedof 15 genes (MYSTI_06004-MYSTI_06018) encoding pol...

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Bibliographic Details
Published inJournal of microbiology and biotechnology Vol. 28; no. 7; pp. 1068 - 1077
Main Authors Hyun, Hyesook, Lee, Sunjin, Lee, Jong Suk, Cho, Kyungyun
Format Journal Article
LanguageEnglish
Published Korea (South) 한국미생물·생명공학회 28.07.2018
Subjects
생물학
Myxococcus stipitatus
DKxanthene
Myxobacteria
biosynthetic gene
secondary metabolite
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Summary:DKxanthenes are a class of yellow secondary metabolites produced by myxobacterial genera and . We identified a putative 49.5 kb DKxanthene biosynthetic gene cluster from DSM 14675 by genomic sequence and mutational analysis. The cluster was comprisedof 15 genes (MYSTI_06004-MYSTI_06018) encoding polyketide synthases, non-ribosomal peptide synthases, and proteins with unknown functions. Disruption of the genes by plasmid insertion resulted in defects in the production of yellow pigments. High-performance liquid chromatography and liquid chromatography-tandem mass spectrometry analysis indicated that the yellow pigments produced by DSM 14675 might be noble DKxanthene derivatives. did not require DKxanthenes for the formation of heat-resistant viable spores, unlike . Furthermore, DKxanthenes showed growth inhibitory activity against the fungi , , and .
ISSN:1017-7825
1738-8872
DOI:10.4014/jmb.1802.02045