Purification and Characterization of a New κ-Carrageenase from the Marine Bacterium Vibrio sp. NJ-2

• Published in: Journal of microbiology and biotechnology Vol. 26; no. 2; pp. 255 - 262
• Main Authors: Zhu, Benwei, Ning, Limin
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 01.02.2016
• Subjects: Bacterial Proteins - chemistry; Bacterial Proteins - isolation & purification; Bacterial Proteins - metabolism; Carrageenan - metabolism; Chromatography, Thin Layer; Culture Media - chemistry; Glycoside Hydrolases - chemistry; Glycoside Hydrolases - isolation & purification; Glycoside Hydrolases - metabolism; Hydrogen-Ion Concentration; Kinetics; Molecular Weight; Oligosaccharides - metabolism; Phylogeny; Rhodophyta - microbiology; Temperature; Vibrio - enzymology; Vibrio - isolation & purification; Vibrio - metabolism; 생물학; characterization; purification; κ-carrageenase; oligosaccharides
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1507.07052

The carrageenan-degrading marine bacterium Vibrio sp. strain NJ-2 was isolated from rotten red algae, and κ-carrageenase with high activity was purified from the culture supernatant. The purified enzyme with molecular mass of 33 kDa showed the maximal activity of 937 U/mg at 40°C and pH 8.0. It maintained 80% of total activity below 40°C and between pH 6.0 and 10.0. The kinetics experiment showed the Km and Vmax values were 2.54 mg/ml and 138.89 mmol/min/mg, respectively. The thin layer chromatography and ESI-MS analysis of hydrolysates indicated that the enzyme can endolytically depolymerize the kappa-carrageenan into oligosaccharides with degrees of depolymerization of 2-8. Owing to its high activity, it could be a valuable tool to produce κ-carrageenan oligosaccharides with various biological activities.