Simple and Rapid Liquid Chromatography-Tandem Mass Spectrometry Analysis of Arctigenin and its Application to a Pharmacokinetic Study

• Published in: Mass spectrometry letters Vol. 8; no. 2; pp. 23 - 28
• Main Authors: Subindra Kazi Thapa, Kwon-yeon Weon, Seok Won Jeong, Tae Hwan Kim, Mahesh Upadhyay, Yo-han Han, Jong-sik Jin, Seung-heon Hong, Yu Seok Youn, Beom Soo Shin, Soyoung Shin
• Format: Journal Article
• Language: English
• Published: 한국질량분석학회 01.06.2017; 사단법인 한국질량분석학회
• Subjects: 화학; Arctigenin; pharmacokinetics; LC-MS/MS; Fructus Arctii
• ISSN: 2233-4203; 2093-8950
• DOI: 10.5478/MSL.2017.8.2.23

Arctigenin is the main active ingredient of Fructus Arctii, which has been reported with a variety of therapeutic activities including anti-cancer, anti-inflammation, anti-virus, and anti-obesity effects. In this study, a simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of arctigenin in rat plasma. The assay utilized a simple protein precipitation with methanol and the mobile phase consisted of 100% methanol and water containing 0.1% formic acid (65:35 v/v). Arctigenin and the internal standard (psoralen) were monitored using a positive electrospray turbo ionspray mode with multiple reaction monitoring transitions of m/z 373.2→136.9 and m/z 187.2→130.9, respectively, and total chromatographic run time was within 5 min. The lower limit of quantification (LLOQ) of arctigenin was 5 ng/mL in the rat plasma. The intra- and inter-day accuracy of arctigenin at LLOQ and matrix-matched quality control samples ranged 97.4 – 104.8% and 97.2 – 102.0%, respectively. The intra-day precision was within 4.80% and the interday precision was within 5.92%. Application of the present method was demonstrated through a pharmacokinetic study after intravenous and oral administration of arctigenin in male Sprague Dawley rats. KCI Citation Count: 0