Antiprotozoal activity of deacetylated chitosan oligosaccharide (dp 2-8) on Trichomonas vaginalis

• Published in: Journal of microbiology and biotechnology Vol. 16; no. 12; pp. 1984 - 1989
• Main Authors: SHIN, Woon-Seob, KIL, Jun-Cheul, PARK, Gab-Man
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.12.2006; 한국미생물·생명공학회
• Subjects: Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; 생물학; Trichomonas vaginalis; Protozoa; chitosan oligosaccharide; Oligosaccharide; Trichomonadida; Chitosan; antitrichomonal agent
• ISSN: 1017-7825

Deacetylated chitosan oligosaccharide (COS) hadeffective antiprotozoal activity against Trichomonas vaginalis(Minimal Inhibitory Concentration, MIC 0.25%), whereas80% acetylated COS showed no antiprotozoal activity (MIC>1%). On the other hand, 80% acetylated COS showedgrowth stimulatory activity against the protozoa. When T.vaginalisconcentration, the viability of the protozoa was rapidly decreasedwithin 15 min, and the protozoa completely died within40 min. Ultrastructural changes of trichomonads treated withCOS included a los of defined nuclear membrane andendoplasmic reticulum membranes, an increase in the numberof free ribosome, vacuolation, and ultimately lysis of the celmembrane. These results indicate that deacetylated COScan be used as an antitrichomonal agent, although its lethalmechanism is not known. KCI Citation Count: 5