Cyanidin-3-glucoside inhibits amyloid β25-35-induced neuronal cell death in cultured rat hippocampal neurons
Increasing evidence implicates changes in [Ca 2+ ] i and oxidative stress as causative factors in amyloid beta (Aβ)-induced neuronal cell death. Cyanidin-3-glucoside (C3G), a component of anthocyanin, has been reported to protect against glutamate-induced neuronal cell death by inhibiting Ca 2+ and...
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Published in | The Korean journal of physiology & pharmacology Vol. 22; no. 6; pp. 689 - 696 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
The Korean Physiological Society and The Korean Society of Pharmacology
01.11.2018
대한약리학회 |
Subjects | |
Online Access | Get full text |
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Summary: | Increasing evidence implicates changes in [Ca
2+
]
i
and oxidative stress as causative factors in amyloid beta (Aβ)-induced neuronal cell death. Cyanidin-3-glucoside (C3G), a component of anthocyanin, has been reported to protect against glutamate-induced neuronal cell death by inhibiting Ca
2+
and Zn
2+
signaling. The present study aimed to determine whether C3G exerts a protective effect against Aβ
25–35
-induced neuronal cell death in cultured rat hippocampal neurons from embryonic day 17 fetal Sprague-Dawley rats using MTT assay for cell survival, and caspase-3 assay and digital imaging methods for Ca
2+
, Zn
2+
, MMP and ROS. Treatment with Aβ
25–35
(20 µM) for 48 h induced neuronal cell death in cultured rat pure hippocampal neurons. Treatment with C3G for 48 h significantly increased cell survival. Pretreatment with C3G for 30 min significantly inhibited Aβ
25–35
-induced [Zn
2+
]
i
increases as well as [Ca
2+
]
i
increases in the cultured rat hippocampal neurons. C3G also significantly inhibited Aβ
25–35
-induced mitochondrial depolarization. C3G also blocked the Aβ
25–35
-induced formation of ROS. In addition, C3G significantly inhibited the Aβ
25–35
-induced activation of caspase-3. These results suggest that cyanidin-3-glucoside protects against amyloid β-induced neuronal cell death by reducing multiple apoptotic signals. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1226-4512 2093-3827 |
DOI: | 10.4196/kjpp.2018.22.6.689 |