Frequency of the three STR loci (TPOX, CSF1PO, THo1), in a Japanese population determined using a Gene Print STR multiplex kit

Allele and genotype frequencies for three tetrametric short tandem repeat loci (TPOX, CSF1PO and TH01) were determined in a Japanese population sample (n = 105) by means of the Gene Print STR Multiplex system PCR kit, electrophoresis of the PCR products in denaturing gels and detection of allelic fr...

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Bibliographic Details
Published inNihon hoigaku zasshi = The Japanese journal of legal medicine Vol. 50; no. 3; p. 163
Main Authors Yamaguchi, H, Takizawa, H, Shimasaki, C
Format Journal Article
LanguageEnglish
Published Japan 01.06.1996
Subjects
Alleles
Electrophoresis
Gene Frequency
Genetics, Population
Genotype
Humans
Japan
Polymerase Chain Reaction - methods
Repetitive Sequences, Nucleic Acid - genetics
Sampling Studies
Silver Staining
Japan
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Summary:Allele and genotype frequencies for three tetrametric short tandem repeat loci (TPOX, CSF1PO and TH01) were determined in a Japanese population sample (n = 105) by means of the Gene Print STR Multiplex system PCR kit, electrophoresis of the PCR products in denaturing gels and detection of allelic fragments by silver staining. Six alleles each were found for the TPOX, CSF1PO, and TH01 loci and at the TPOX locus, we identified an additional mutation allele, which consisted of 7 AATG repeats. The TPOX, CSF1PO and TH01 loci were apparently 64, 73, and 74% heterozygous, respectively. All loci met the Hardy-Weinberg criteria using categorized alleles.
ISSN:0047-1887