QuEChERS를 이용한 원두커피로부터 다환방향족탄화수소 4종(PAH4)의 신속 추출 및 분석

• Published in: 한국식품과학회지, 57(2) pp. 134 - 141
• Main Authors: 정희정, 신재민, 최희진, 김다희, 손여준, 장미라, 황인숙, 김선아
• Format: Journal Article
• Language: Korean
• Published: 한국식품과학회 01.04.2025
• Subjects: 식품과학
• ISSN: 0367-6293
• DOI: 10.9721/KJFST.2025.57.2.134

This study aims to establish and validate a rapid analytical method using QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) pretreatment to analyze four polycyclic aromatic hydrocarbons (PAHs) in roasted coffee beans. In addition, the PAH4 content of 50 commercially available coffee bean samples was investigated and human exposure was assessed. Comparing the European Norm (EN) and Association of Official Analytical Chemists (AOAC) methods in the QuEChERS pretreatment, both methods showed similar levels of impurity removal and recovery. The EN method was selected because of its better phase separation during the extraction and purification process. The validation of the QuEChERS method showed good linearity and correlation coefficients (R2), with limit of quantification (LOQ) values ranging from 0.15 to 1.86 μg/kg. The method also exhibited high accuracy and precision, falling within the acceptable range of the CODEX codex alimentarius commission (CAC) guidelines. Among 28 whole bean coffee and 22 capsule coffee, Benz[a]anthracene was detected in 19 samples (38%) at concentrations ranging from ND to 7.99 μg/kg, Chrysene in 45 samples (90%) from ND to 13.78 μg/kg, Benzo[b]fluoranthene in 34 samples (68%) from ND to 20.31 μg/ kg, and Benzo[a]pyrene in 13 samples (26%) from ND to 3.09 μg/kg. Based on these PAH4 concentrations, the estimated human exposure was 2.165 ng/kg bw/day, which is lower than the exposure level to PAH4 through food intake. KCI Citation Count: 0