Compatibility of Neural Stem Cells with Functionalized Selfassembling Peptide Scaffold In vitro

• Published in: Biotechnology and bioprocess engineering pp. 545 - 551
• Main Authors: Zhen Xing Zhang, Ding Jun Hao, Qi Xin Zheng, Yong Chao Wu
• Format: Journal Article
• Language: English
• Published: 한국생물공학회 01.08.2010
• Subjects: 생물공학
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-009-3076-2

In this study, we evaluated the behavior of neural stem cells (NSCs) using a new peptide hydrogel scaffold named IKVAVmx, which was made by mixing self-assembling peptide RADA16 and designer peptide RADA16-IKVAV solutions. NSCs derived from rat cerebral cortex were culture-expanded in neuorobasal medium and seeded on the RADA16 and IKVAVmx hydrogels. Cells could penetrate the hydrogels and form a 3D cellular network. Compared to pure RADA16 scaffold, we found that IKVAVmx scaffold significantly promoted cell proliferation and stimulated cell migration into the 3D scaffold. Moreover, Immunocytochemistry and Western blot analysis indicated that the differentiation ratio of neurons from NSCs in IKVAVmx scaffold was higher than that in pure RADA16 scaffold. These results suggested that this new hydrogel scaffold provided an ideal substrate for NSCs 3D culture and suggested its further application for neural tissue engineering. KCI Citation Count: 25