Statistical Optimization of Medium Components for the Production of Xylanase by Aspergillus niger KK2 in Submerged Cultivation

• Published in: Biotechnology and bioprocess engineering pp. 302 - 307
• Main Authors: 김성욱, 박철환, 김찬화, Byeong Jo Min, Yang Soon Park, Seong Woo Kang, 송윤석, 이종호
• Format: Journal Article
• Language: English
• Published: 한국생물공학회 01.06.2007
• Subjects: 생물공학
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/BF02931108

Medium composition was optimized for the production of xylanase by Aspergillus niger KK2 using statistical experimental designs. Corn steep liquor (CSL) and industrial yeast extract (IYE) were the most important factors affecting xylanase activity. The medium that produced the optimum conditions for the production of xylanase contained 3% rice straw, 1% wheat bran, 6.3% CSL, 0.15% IYE, and 0.5% KH2PO4. After 4 days of cultivation under optimized conditions in a 2.5-L stirred tank reactor, the activity and productivity of xylanase were 620 IU/mL and 6,458 IU/L·h, respectively. The highest xylanase activity obtained using the optimized medium was 80% greater than the activity obtained using basal medium. The xylanase activity predicted by a polynomial model was 670 IU/mL. KCI Citation Count: 13