Reovirus Strain-Dependent Inflammatory Cytokine Responses and Replication Patterns in a Human Monocyte Cell Line
Mammalian Orthoreoviruses are important models for studies of viral pathogenesis. In the rat lung, Reovirus strain type 3 Dearing (T3D) induces substantially more inflammation than does strain type 1 Lang (T1L). To better understand mechanisms underlying differences in the host inflammatory response...
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Published in | Viral Immunology Vol. 19; no. 3; pp. 546 - 557 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Mary Ann Liebert, Inc
01.09.2006
|
Subjects | |
Online Access | Get full text |
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Summary: | Mammalian Orthoreoviruses are important models for studies of viral pathogenesis. In the rat lung,
Reovirus strain type 3 Dearing (T3D) induces substantially more inflammation than does strain type
1 Lang (T1L). To better understand mechanisms underlying differences in the host inflammatory
response elicited by T1L and T3D, we characterized cytokine expression patterns induced by those
strains after infection of THP-1 monocyte cells. THP-1 cells were adsorbed with either viable or ultraviolet-
inactivated T1L and T3D and assayed for mRNA and protein production of growth-regulated
oncogene-
α
(GRO-
α
), interleukin-8 (IL-8), or tumor necrosis factor-
α
(TNF-
α
). T3D stimulated
mRNA and protein production of all three cytokines, whereas T1L stimulated mRNA and
protein production of IL-8 and TNF-
α
but not GRO-
α
. In each case, T3D induced greater cytokine
mRNA and protein expression than did T1L. Nonviable virus did not stimulate detectable cytokine
secretion, suggesting a requirement for viral RNA synthesis in cytokine induction by THP-1 cells.
A greater percentage of THP-1 cells was infected with T1L than T3D as assessed by infectious center
assay, and T1L achieved higher yields of infectious progeny than did T3D in infected THP-1
cells as determined by plaque assay. These strain-dependent differences in cytokine responses and
corresponding replication patterns in monocyte cells parallel findings made in studies of rat models
of pneumonia and provide clues about how Reovirus interfaces with the host innate immune response
to produce pulmonary disease. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0882-8245 1557-8976 1365-2567 |
DOI: | 10.1089/vim.2006.19.546 |