Drug resistance and cross-sensitivity in cultured human osteosarcoma cells

The sensitivity, cross-sensitivity, acquired resistance and restoration of sensitivity to adriamycin (ADR) in cultured human osteosarcoma (OST) cells were studied from the viewpoints of cell proliferation and cell cycle pattern by flow cytometry. OST cells resistant to ADR were produced by a treatme...

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Bibliographic Details
Published inNippon Seikeigekagakkai zasshi Vol. 63; no. 4; p. 274
Main Authors Tomita, K, Kontani, T, Tsuchiya, H, Nomura, S
Format Journal Article
LanguageEnglish
Published Japan 01.04.1989
Subjects
Cisplatin - pharmacology
Doxorubicin - pharmacology
Drug Resistance
Drug Screening Assays, Antitumor
Humans
Methotrexate - pharmacology
Osteosarcoma - pathology
Tumor Cells, Cultured
Vincristine - pharmacology
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Summary:The sensitivity, cross-sensitivity, acquired resistance and restoration of sensitivity to adriamycin (ADR) in cultured human osteosarcoma (OST) cells were studied from the viewpoints of cell proliferation and cell cycle pattern by flow cytometry. OST cells resistant to ADR were produced by a treatment which consists of a 40-hour exposure to high doses of ADR and subsequent cultivation for 6 weeks and was repeated five times. ADR-resistant cells showed cross-resistance to methotrexate (MTX), and vincristine (VCR), and collateral sensitivity to cisplatin (CDDP). Treatment of the ADR-resistant cells with ADR and verapamil, a Ca-antagonist, resulted in an increased inhibition of sarcoma cell growth.
ISSN:0021-5325