Primed to Naive-Like Conversion of the Common Marmoset Embryonic Stem Cells

Mammalian pluripotent stem cells are thought to exist in two states: naive and primed. Generally, unlike those in rodents, pluripotent stem cells in primates, including humans, are regarded as being in the primed pluripotent state. Recently, several groups reported the existence of naive pluripotent...

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Published inStem cells and development Vol. 29; no. 12; p. 761
Main Authors Shiozawa, Seiji, Nakajima, Mayutaka, Okahara, Junko, Kuortaki, Yoko, Kisa, Fumihiko, Yoshimatsu, Sho, Nakamura, Mari, Koya, Ikuko, Yoshimura, Mika, Sasagawa, Yohei, Nikaido, Itoshi, Sasaki, Erika, Okano, Hideyuki
Format Journal Article
LanguageEnglish
Published United States 15.06.2020
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Abstract Mammalian pluripotent stem cells are thought to exist in two states: naive and primed. Generally, unlike those in rodents, pluripotent stem cells in primates, including humans, are regarded as being in the primed pluripotent state. Recently, several groups reported the existence of naive pluripotent stem cells in humans. In this study, we report the conversion of primed state embryonic stem cells from common marmoset, a New World monkey, to the naive state using transgenes. The cells showed typical naive state features, including dome-like colony morphology, growth factor requirement, gene expression profile, X chromosome activation state, and energy metabolic status. Moreover, interspecies chimeric embryo formation ability with mouse embryos was increased in the naive state. This technique can be applied in basic medical research using nonhuman primates, such as preclinical use of naive pluripotent stem cells and generating genetically modified primates.
AbstractList Mammalian pluripotent stem cells are thought to exist in two states: naive and primed. Generally, unlike those in rodents, pluripotent stem cells in primates, including humans, are regarded as being in the primed pluripotent state. Recently, several groups reported the existence of naive pluripotent stem cells in humans. In this study, we report the conversion of primed state embryonic stem cells from common marmoset, a New World monkey, to the naive state using transgenes. The cells showed typical naive state features, including dome-like colony morphology, growth factor requirement, gene expression profile, X chromosome activation state, and energy metabolic status. Moreover, interspecies chimeric embryo formation ability with mouse embryos was increased in the naive state. This technique can be applied in basic medical research using nonhuman primates, such as preclinical use of naive pluripotent stem cells and generating genetically modified primates.
Author Okahara, Junko
Yoshimura, Mika
Nakajima, Mayutaka
Kisa, Fumihiko
Koya, Ikuko
Yoshimatsu, Sho
Shiozawa, Seiji
Nakamura, Mari
Nikaido, Itoshi
Kuortaki, Yoko
Sasagawa, Yohei
Sasaki, Erika
Okano, Hideyuki
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  surname: Shiozawa
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  organization: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
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  givenname: Mayutaka
  surname: Nakajima
  fullname: Nakajima, Mayutaka
  organization: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
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  givenname: Junko
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  organization: Department of Marmoset Biology and Medicine, Central Institute for Experimental Animals, Kawasaki, Japan
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  givenname: Yoko
  surname: Kuortaki
  fullname: Kuortaki, Yoko
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  givenname: Fumihiko
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  organization: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
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  organization: Department of Physiology, School of Medicine, Keio University, Tokyo, Japan
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  surname: Yoshimura
  fullname: Yoshimura, Mika
  organization: Laboratory for Bioinformatics Research, RIKEN Center for Biosystems Dynamics Research, Wako, Japan
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  givenname: Yohei
  surname: Sasagawa
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  givenname: Itoshi
  surname: Nikaido
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  givenname: Erika
  surname: Sasaki
  fullname: Sasaki, Erika
  organization: Department of Marmoset Biology and Medicine, Central Institute for Experimental Animals, Kawasaki, Japan
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  givenname: Hideyuki
  surname: Okano
  fullname: Okano, Hideyuki
  organization: Laboratory for Marmoset Neural Architecture, RIKEN Center for Brain Science, Wako, Japan
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Keywords embryonic stem cell
naive pluripotency
common marmoset
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Snippet Mammalian pluripotent stem cells are thought to exist in two states: naive and primed. Generally, unlike those in rodents, pluripotent stem cells in primates,...
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SubjectTerms Animals
Callithrix
Cell Line
Cell Shape
Chimera - genetics
Chimera - metabolism
Embryonic Stem Cells - cytology
Embryonic Stem Cells - metabolism
Energy Metabolism
Genetic Engineering - methods
Transcriptome
Transgenes
X Chromosome Inactivation
Title Primed to Naive-Like Conversion of the Common Marmoset Embryonic Stem Cells
URI https://www.ncbi.nlm.nih.gov/pubmed/32188344
Volume 29
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