Primed to Naive-Like Conversion of the Common Marmoset Embryonic Stem Cells

Mammalian pluripotent stem cells are thought to exist in two states: naive and primed. Generally, unlike those in rodents, pluripotent stem cells in primates, including humans, are regarded as being in the primed pluripotent state. Recently, several groups reported the existence of naive pluripotent...

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Published inStem cells and development Vol. 29; no. 12; p. 761
Main Authors Shiozawa, Seiji, Nakajima, Mayutaka, Okahara, Junko, Kuortaki, Yoko, Kisa, Fumihiko, Yoshimatsu, Sho, Nakamura, Mari, Koya, Ikuko, Yoshimura, Mika, Sasagawa, Yohei, Nikaido, Itoshi, Sasaki, Erika, Okano, Hideyuki
Format Journal Article
LanguageEnglish
Published United States 15.06.2020
Subjects
Animals
Callithrix
Cell Line
Cell Shape
Chimera - genetics
Chimera - metabolism
Embryonic Stem Cells - cytology
Embryonic Stem Cells - metabolism
Energy Metabolism
Genetic Engineering - methods
Transcriptome
Transgenes
X Chromosome Inactivation
embryonic stem cell
naive pluripotency
common marmoset
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Summary:Mammalian pluripotent stem cells are thought to exist in two states: naive and primed. Generally, unlike those in rodents, pluripotent stem cells in primates, including humans, are regarded as being in the primed pluripotent state. Recently, several groups reported the existence of naive pluripotent stem cells in humans. In this study, we report the conversion of primed state embryonic stem cells from common marmoset, a New World monkey, to the naive state using transgenes. The cells showed typical naive state features, including dome-like colony morphology, growth factor requirement, gene expression profile, X chromosome activation state, and energy metabolic status. Moreover, interspecies chimeric embryo formation ability with mouse embryos was increased in the naive state. This technique can be applied in basic medical research using nonhuman primates, such as preclinical use of naive pluripotent stem cells and generating genetically modified primates.
ISSN:1557-8534
DOI:10.1089/scd.2019.0259