Salmonella Typhimurium oral challenge model in mature broilers: bacteriological, immunological, and growth performance aspects

• Published in: Poultry science Vol. 90; no. 1; pp. 59 - 67
• Main Authors: Marcq, C, Cox, E, Szalo, I M, Théwis, A, Beckers, Y
• Format: Journal Article Web Resource
• Language: English
• Published: England Poultry Science Association 01.01.2011
• Subjects: Animal production & animal husbandry; Animals; Antibodies, Bacterial - physiology; Bacteria; chicken; Chickens; immune response; Immunoglobulin A; Immunoglobulins; intestinal colonization; Intestines - immunology; Intestines - microbiology; Life sciences; Male; mature broiler; Microbiologie; Microbiology; Médecine vétérinaire & santé animale; Poultry Diseases - microbiology; Productions animales & zootechnie; Salmonella enterica; Salmonella enterica serovar Typhimurium; Salmonella Infections, Animal - microbiology; Salmonella typhimurium; Salmonella typhimurium - physiology; Sciences du vivant; Veterinary medicine & animal health
• ISSN: 0032-5791; 1525-3171
• DOI: 10.3382/ps.2010-01017

In this study, Salmonella enterica serovar Typhimurium challenge models were tested to identify the best conditions under which to perform the experimental infection of 3-wk-old broilers. Such a model would be useful to study the efficiency of therapeutic treatments that could take place at the end of the grow-out period. Salmonella-free chicks were obtained from a breeder flock vaccinated with Salmonella. Intestinal maternal immunity was monitored by ELISA analyses at 2, 9, and 16 d of age. Data indicated that protection of maternal origin was not maintained over time and was drastically reduced at 9 d of age (P < 0.01). At 21 d of age, chickens were orally inoculated with Salmonella Typhimurium. The effects of the oral challenge dose (0, 3 × 10(3), 3 × 10(6), and 3 × 10(9) cfu/bird) and vancomycin pretreatment (no administration or 25 mg/bird) on intestinal immune responses, growth performance, and Salmonella colonization of chickens were investigated. After infection, the mucosal immune response was rapid, with increased (P < 0.01) anti-Salmonella Typhimurium IgA titers measured at 8 d postinfection in intestinal contents. A linear relationship (P < 0.05) existed between specific IgA levels in intestinal and cecal contents and the challenge dose inoculated. None of the challenge protocols caused mortality or clinical symptoms after infection. Nevertheless, the experimental infection induced a significant deterioration of growth performance. The pretreatment with 25 mg of vancomycin at 3 h before Salmonella inoculation was able to establish stable infection rates among the population of 3-wk-old infected chickens. Nevertheless, Salmonella shedding was not stable over the rearing period, and the bacteria seemed to be naturally eliminated from most birds at 22 d postinfection. This natural clearance of the gut, which was related, at least in part, to the intestinal immune response, should limit the usability of the created mature challenge model within 1 to 2 wk after inoculation.