Combined enzymatic hydrolysis and HPAEC method for simultaneous analysis of galacturonic acid and neutral sugars of pectin

Pectic substances are heteropolysaccharides from plant cell walls, which mainly consist of a homogalacturonan backbone of predominantly alpha-(1-->4) linked galacturonic acid (GalA) residues. This chain is interrupted by ramified rhamnogalacturonan regions with a certain amount of neutral sugars...

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Bibliographic Details
Published inCommunications in agricultural and applied biological sciences Vol. 68; no. 2 Pt A; p. 297
Main Authors Garna, H, Mabon, N, Wathelet, B, Paquot, M
Format Journal Article
LanguageEnglish
Published Belgium 2003
Subjects
Carbohydrates - analysis
Carbohydrates - isolation & purification
Chromatography, Gas
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Hexuronic Acids - analysis
Hexuronic Acids - isolation & purification
Hydrolysis
Malus
Pectins - chemistry
Pectins - metabolism
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Summary:Pectic substances are heteropolysaccharides from plant cell walls, which mainly consist of a homogalacturonan backbone of predominantly alpha-(1-->4) linked galacturonic acid (GalA) residues. This chain is interrupted by ramified rhamnogalacturonan regions with a certain amount of neutral sugars (rhamnose, arabinose, galactose, glucose, xylose and mannose) present as side-chains. The GalA residues can be methyl-esterified at the carboxyl group. Usually uronic acids of pectin are determined as anhydrogalacturonic acid by spectrophotometry, using the metahydrodiphenyl method (Thibault, 1979), while the monosaccharides are determined by High-Performance Liquid Chromatography (HPLC) after a methanolysis (Quemener and Thibault, 1990) or by Gaz Liquid Chromatography (GLC) as alditol acetates according to Blakeney et al. (1983). In this paper, a combined enzymatic hydrolysis and HPLC method is validated for simultaneous analysis of galacturonic acid and neutral sugars without any derivatisation.
ISSN:1379-1176